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Immunohistochemical Expression of TGF-β1 and Osteonectin in engineered and Ca(OH)2-repaired human pulp tissues

Overview of attention for article published in Brazilian Oral Research, October 2016
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Title
Immunohistochemical Expression of TGF-β1 and Osteonectin in engineered and Ca(OH)2-repaired human pulp tissues
Published in
Brazilian Oral Research, October 2016
DOI 10.1590/1807-3107bor-2016.vol30.0093
Pubmed ID
Authors

CHISINI, Luiz Alexandre, CONDE, Marcus Cristian Muniz, ALCÁZAR, Jose Carlos Bernedo, SILVA, Adriana Fernandes da, NÖR, Jacques Eduardo, TARQUINIO, Sandra Beatriz Chaves, DEMARCO, Flávio Fernando

Abstract

The aim of the present study was to evaluate the expression of transforming growth factor-β1 (TGF-β1) and osteonectin (ON) in pulp-like tissues developed by tissue engineering and to compare it with the expression of these proteins in pulps treated with Ca(OH)2 therapy. Tooth slices were obtained from non-carious human third molars under sterile procedures. The residual periodontal and pulp soft tissues were removed. Empty pulp spaces of the tooth slice were filled with sodium chloride particles (250-425 µm). PLLA solubilized in 5% chloroform was applied over the salt particles. The tooth slice/scaffold (TS/S) set was stored overnight and then rinsed thoroughly to wash out the salt. Scaffolds were previously sterilized with ethanol (100-70°) and washed with phosphate-buffered saline (PBS). TS/S was treated with 10% EDTA and seeded with dental pulp stem cells (DPSC). Then, TS/S was implanted into the dorsum of immunodeficient mice for 28 days. Human third molars previously treated with Ca(OH)2 for 90 days were also evaluated. Samples were prepared and submitted to histological and immunohistochemical (with anti-TGF-β1, 1:100 and anti-ON, 1:350) analyses. After 28 days, TS/S showed morphological characteristics similar to those observed in dental pulp treated with Ca(OH)2. Ca(OH)2-treated pulps showed the usual repaired pulp characteristics. In TS/S, newly formed tissues and pre-dentin was colored, which elucidated the expression of TGF-β1 and ON. Immunohistochemistry staining of Ca(OH)2-treated pulps showed the same expression patterns. The extracellular matrix displayed a fibrillar pattern under both conditions. Regenerative events in the pulp seem to follow a similar pattern of TGF-β1 and ON expression as the repair processes.

Mendeley readers

The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 41 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 10 24%
Student > Bachelor 7 17%
Professor 4 10%
Student > Postgraduate 3 7%
Student > Ph. D. Student 2 5%
Other 5 12%
Unknown 10 24%
Readers by discipline Count As %
Medicine and Dentistry 22 54%
Biochemistry, Genetics and Molecular Biology 3 7%
Nursing and Health Professions 2 5%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Agricultural and Biological Sciences 1 2%
Other 2 5%
Unknown 10 24%