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Flow rate dependent continuous hydrolysis of protein isolates

Overview of attention for article published in AMB Express, February 2018
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Title
Flow rate dependent continuous hydrolysis of protein isolates
Published in
AMB Express, February 2018
DOI 10.1186/s13568-018-0548-9
Pubmed ID
Authors

Tim Sewczyk, Marieke Hoog Antink, Michael Maas, Stephen Kroll, Sascha Beutel

Abstract

Food protein hydrolysates are often produced in unspecific industrial batch processes. The hydrolysates composition underlies process-related fluctuations and therefore the obtained peptide fingerprint and bioactive properties may vary. To overcome this obstacle and enable the production of specific hydrolysates with selected peptides, a ceramic capillary system was developed and characterized for the continuous production of a consistent peptide composition. Therefore, the protease Alcalase was immobilized on the surface of aminosilane modified yttria stabilized zirconia capillaries with a pore size of 1.5 µm. The loading capacity was 0.3 µg enzyme per mg of capillary with a residual enzyme activity of 43%. The enzyme specific peptide fingerprint produced with this proteolytic capillary reactor system correlated with the degree of hydrolysis, which can be controlled over the residence time by adjusting the flow rate. Common food proteins like casein, sunflower and lupin protein isolates were tested for continuous hydrolysis in the developed reactor system. The peptide formation was investigated by high-performance liquid chromatography. Various trends were found for the occurrence of specific peptides. Some are just intermediately occurring, while others cumulate by time. Thus, the developed continuous reactor system enables the production of specific peptides with desired bioactive properties.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 33 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 18%
Student > Ph. D. Student 5 15%
Student > Master 4 12%
Student > Doctoral Student 3 9%
Researcher 2 6%
Other 2 6%
Unknown 11 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 15%
Medicine and Dentistry 3 9%
Chemical Engineering 3 9%
Environmental Science 2 6%
Agricultural and Biological Sciences 2 6%
Other 6 18%
Unknown 12 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 February 2018.
All research outputs
#15,492,327
of 23,023,224 outputs
Outputs from AMB Express
#447
of 1,241 outputs
Outputs of similar age
#272,616
of 444,262 outputs
Outputs of similar age from AMB Express
#12
of 47 outputs
Altmetric has tracked 23,023,224 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,241 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 444,262 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 47 others from the same source and published within six weeks on either side of this one. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.