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Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid

Overview of attention for article published in FEMS Yeast Research, February 2018
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#14 of 989)
  • High Attention Score compared to outputs of the same age (89th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Mentioned by

twitter
19 tweeters
patent
1 patent
facebook
2 Facebook pages
wikipedia
2 Wikipedia pages

Citations

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56 Dimensions

Readers on

mendeley
145 Mendeley
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Title
Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid
Published in
FEMS Yeast Research, February 2018
DOI 10.1093/femsyr/foy012
Pubmed ID
Authors

Hannes Juergens, Javier A Varela, Arthur R Gorter de Vries, Thomas Perli, Veronica J M Gast, Nikola Y Gyurchev, Arun S Rajkumar, Robert Mans, Jack T Pronk, John P Morrissey, Jean-Marc G Daran

Abstract

While CRISPR-Cas9-mediated genome editing has transformed yeast research, current plasmids and cassettes for Cas9 and guide-RNA expression are species specific. CRISPR tools that function in multiple yeast species could contribute to the intensifying research on non-conventional yeasts. A plasmid carrying a pangenomic origin of replication and two constitutive expression cassettes, for Cas9 and a ribozyme-flanked gRNAs was constructed. Its functionality was tested by analyzing inactivation of the ADE2 gene in four yeast species. In two Kluyveromyces species, near-perfect targeting ( ≥ 96%) and homologous repair (HR) was observed in at least 24% of transformants. In two Ogataea species, Ade- mutants were not observed directly after transformation, but prolonged incubation of transformed cells resulted in targeting efficiencies of 9 to 63% mediated by non-homologous end joining (NHEJ). In a O. parapolymorpha KU80 mutant, deletion of OpADE2 mediated by HR was achieved, albeit at low efficiencies ( < 1%). Furthermore the expression of a dual polycistronic gRNA-array enabled simultaneous interruption of OpADE2 and OpYNR1 demonstrating flexibility of ribozyme-flanked gRNA design for multiplexing. While prevalence of NHEJ prevented HR-mediated editing in Ogataea, such targeted editing was possible in Kluyveromyces. This broad-host-range CRISPR/gRNA system may contribute to exploration of Cas9-mediated genome editing in other Saccharomycotina yeasts.

Twitter Demographics

The data shown below were collected from the profiles of 19 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 145 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 145 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 25 17%
Student > Master 24 17%
Researcher 20 14%
Student > Bachelor 12 8%
Student > Doctoral Student 7 5%
Other 18 12%
Unknown 39 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 65 45%
Agricultural and Biological Sciences 17 12%
Engineering 7 5%
Immunology and Microbiology 5 3%
Chemical Engineering 4 3%
Other 7 5%
Unknown 40 28%

Attention Score in Context

This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 November 2020.
All research outputs
#1,498,739
of 20,902,589 outputs
Outputs from FEMS Yeast Research
#14
of 989 outputs
Outputs of similar age
#41,017
of 400,359 outputs
Outputs of similar age from FEMS Yeast Research
#1
of 17 outputs
Altmetric has tracked 20,902,589 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 989 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 400,359 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.