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The FXXLF Motif Mediates Androgen Receptor-specific Interactions with Coregulators

Overview of attention for article published in Journal of Biological Chemistry, March 2002
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Title
The FXXLF Motif Mediates Androgen Receptor-specific Interactions with Coregulators
Published in
Journal of Biological Chemistry, March 2002
DOI 10.1074/jbc.m111975200
Pubmed ID
Authors

Bin He, John T. Minges, Lori W. Lee, Elizabeth M. Wilson

Abstract

The androgen receptor (AR) activation function 2 region of the ligand binding domain binds the LXXLL motifs of p160 coactivators weakly, engaging instead in an androgen-dependent, interdomain interaction with an FXXLF motif in the AR NH(2) terminus. Here we show that FXXLF motifs are present in previously reported AR coactivators ARA70/RFG, ARA55/Hic-5, and ARA54, which account for their selection in yeast two-hybrid screens. Mammalian two-hybrid assays, ligand dissociation rate studies, and glutathione S-transferase adsorption assays indicate androgen-dependent selective interactions of these FXXLF motifs with the AR ligand binding domain. Mutagenesis of residues within activation function 2 indicates distinct but overlapping binding sites where specificity depends on sequences within and flanking the FXXLF motif. Mutagenesis of the FXXLF motifs eliminated interaction with the ligand binding domain but only modestly reduced AR coactivation in transcription assays. The studies indicate that the FXXLF binding motif is specific for the AR and mediates interactions both within the AR and with coregulatory proteins.

Mendeley readers

The data shown below were compiled from readership statistics for 56 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 2%
Spain 1 2%
Unknown 54 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 30%
Student > Master 10 18%
Researcher 7 13%
Student > Doctoral Student 5 9%
Professor 3 5%
Other 9 16%
Unknown 5 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 29%
Biochemistry, Genetics and Molecular Biology 14 25%
Chemistry 9 16%
Medicine and Dentistry 6 11%
Pharmacology, Toxicology and Pharmaceutical Science 2 4%
Other 1 2%
Unknown 8 14%