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Production of (2R, 3R)-2,3-butanediol using engineered Pichia pastoris: strain construction, characterization and fermentation

Overview of attention for article published in Biotechnology for Biofuels, February 2018
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Title
Production of (2R, 3R)-2,3-butanediol using engineered Pichia pastoris: strain construction, characterization and fermentation
Published in
Biotechnology for Biofuels, February 2018
DOI 10.1186/s13068-018-1031-1
Pubmed ID
Authors

Zhiliang Yang, Zisheng Zhang

Abstract

2,3-butanediol (2,3-BD) is a bulk platform chemical with various potential applications such as aviation fuel. 2,3-BD has three optical isomers: (2R, 3R)-, (2S, 3S)- and meso-2,3-BD. Optically pure 2,3-BD is a crucial precursor for the chiral synthesis and it can also be used as anti-freeze agent due to its low freezing point. 2,3-BD has been produced in both native and non-native hosts. Several pathogenic bacteria were reported to produce 2,3-BD in mixture of its optical isomers includingKlebsiella pneumoniaeandKlebsiella oxytoca. Engineered hosts based on episomal plasmid expression such asEscherichia coli,Saccharomyces cerevisiaeandBacillus subtilisare not ideal for industrial fermentation due to plasmid instability. Pichia pastoris is generally regarded as safe and a well-established host for high-level heterologous protein production. To produce pure (2R, 3R)-2,3-BD enantiomer, we developed aP. pastorisstrain by introducing a synthetic pathway. ThealsS andalsD genes fromB. subtiliswere codon-optimized and synthesized. The BDH1 gene fromS. cerevisiaewas cloned. These three pathway genes were integrated into the genome ofP. pastorisand expressed under the control of GAP promoter. Production of (2R, 3R)-2,3-BD was achieved using glucose as feedstock. The optical purity of (2R, 3R)-2,3-BD was more than 99%. The titer of (2R, 3R)-2,3-BD reached 12 g/L with 40 g/L glucose as carbon source in shake flask fermentation. The fermentation conditions including pH, agitation speeds and aeration rates were optimized in batch cultivations. The highest titer of (2R, 3R)-2,3-BD achieved in fed-batch fermentation using YPD media was 45 g/L. The titer of 2,3-BD was enhanced to 74.5 g/L through statistical medium optimization. The potential of engineeringP. pastorisinto a microbial cell factory for biofuel production was evaluated in this work using (2R, 3R)-2,3-BD as an example. EngineeredP. pastoriscould be a promising workhorse for the production of optically pure (2R, 3R)-2,3-BD.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 20%
Student > Bachelor 4 16%
Student > Ph. D. Student 4 16%
Student > Master 3 12%
Student > Doctoral Student 2 8%
Other 2 8%
Unknown 5 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 40%
Agricultural and Biological Sciences 5 20%
Chemical Engineering 2 8%
Arts and Humanities 1 4%
Engineering 1 4%
Other 0 0%
Unknown 6 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 February 2018.
All research outputs
#10,019,891
of 12,521,852 outputs
Outputs from Biotechnology for Biofuels
#715
of 963 outputs
Outputs of similar age
#201,428
of 270,258 outputs
Outputs of similar age from Biotechnology for Biofuels
#12
of 14 outputs
Altmetric has tracked 12,521,852 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 963 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 15th percentile – i.e., 15% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 270,258 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one is in the 7th percentile – i.e., 7% of its contemporaries scored the same or lower than it.