| Chapter title |
Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing
|
|---|---|
| Chapter number | 20 |
| Book title |
Sertoli Cells
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7698-0_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7697-3, 978-1-4939-7698-0
|
| Authors |
Yoni Baert, Jaime Onofre, Dorien Van Saen, Ellen Goossens, Baert, Yoni, Onofre, Jaime, Saen, Dorien Van, Goossens, Ellen |
| Abstract |
Tissue cryopreservation uses very low temperatures to preserve structurally intact living cells in their natural microenvironment. Cell survival is strongly influenced by the biophysical effects of ice during both the freezing and the subsequent thawing. These effects can be controlled by optimizing the fragment size, type of cryoprotectant, and cooling rate. The challenge is to determine cryopreservation parameters that suit all cell types present in the tissue. Here we describe a quick and convenient protocol for the cryopreservation of testicular tissue using an isopropyl-insulated freezing device, which was validated in both a mouse and a human model. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 19% |
| Researcher | 3 | 19% |
| Student > Master | 3 | 19% |
| Student > Bachelor | 2 | 13% |
| Unspecified | 1 | 6% |
| Other | 3 | 19% |
| Unknown | 1 | 6% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 38% |
| Medicine and Dentistry | 3 | 19% |
| Agricultural and Biological Sciences | 2 | 13% |
| Nursing and Health Professions | 1 | 6% |
| Unspecified | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 2 | 13% |