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Sertoli Cells

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Cover of 'Sertoli Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Establishment of Primary Culture of Sertoli Cells
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    Chapter 2 Evaluation of the Purity of Sertoli Cell Primary Cultures
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    Chapter 3 Preparation of Testicular Samples for Histology and Immunohistochemistry
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    Chapter 4 Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age
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    Chapter 5 Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy
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    Chapter 6 Sertoli Cell Preparation for Co-immunoprecipitation
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    Chapter 7 Profiling Signaling Proteins in Sertoli Cells by Co-immunoprecipitation
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    Chapter 8 Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy
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    Chapter 9 A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells
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    Chapter 10 Assessing Autophagy in Sertoli Cells
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    Chapter 11 Molecular Mechanisms and Signaling Pathways Involved in the Nutritional Support of Spermatogenesis by Sertoli Cells
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    Chapter 12 Assessing Sertoli Cell Metabolic Activity
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    Chapter 13 Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy
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    Chapter 14 Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs
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    Chapter 15 Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice
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    Chapter 16 Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of Actin-, and Microtubule-Based Cytoskeletons
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    Chapter 17 Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay
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    Chapter 18 Computational Methods Involved in Evaluating the Toxicity of the Reproductive Toxicants in Sertoli Cell
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    Chapter 19 A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells
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    Chapter 20 Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing
Attention for Chapter 6: Sertoli Cell Preparation for Co-immunoprecipitation
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Chapter title
Sertoli Cell Preparation for Co-immunoprecipitation
Chapter number 6
Book title
Sertoli Cells
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7698-0_6
Pubmed ID
Book ISBNs
978-1-4939-7697-3, 978-1-4939-7698-0
Authors

Maria João Freitas, Margarida Fardilha, Freitas, Maria João, Fardilha, Margarida

Abstract

Most techniques to study protein-protein interactions, gene expression or signal transduction, among others, in Sertoli cells, depend on obtaining a protein extract of such cells. This is accomplished by lysing the Sertoli cells and solubilizing the intracellular proteins. Depending on the purpose of your study, the technique used to lyse and consequently obtain protein extracts from Sertoli cells must be considered. In this chapter, we will focus on how to obtain a protein Sertoli cell extract suitable for co-immunoprecipitation technique.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 1 25%
Unspecified 1 25%
Student > Ph. D. Student 1 25%
Unknown 1 25%
Readers by discipline Count As %
Unspecified 1 25%
Biochemistry, Genetics and Molecular Biology 1 25%
Immunology and Microbiology 1 25%
Unknown 1 25%