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Mendeley readers
Chapter title |
Sertoli Cell Preparation for Co-immunoprecipitation
|
---|---|
Chapter number | 6 |
Book title |
Sertoli Cells
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7698-0_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7697-3, 978-1-4939-7698-0
|
Authors |
Maria João Freitas, Margarida Fardilha, Freitas, Maria João, Fardilha, Margarida |
Abstract |
Most techniques to study protein-protein interactions, gene expression or signal transduction, among others, in Sertoli cells, depend on obtaining a protein extract of such cells. This is accomplished by lysing the Sertoli cells and solubilizing the intracellular proteins. Depending on the purpose of your study, the technique used to lyse and consequently obtain protein extracts from Sertoli cells must be considered. In this chapter, we will focus on how to obtain a protein Sertoli cell extract suitable for co-immunoprecipitation technique. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 1 | 25% |
Unspecified | 1 | 25% |
Student > Ph. D. Student | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 25% |
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Immunology and Microbiology | 1 | 25% |
Unknown | 1 | 25% |