| Chapter title |
Evaluation of the Purity of Sertoli Cell Primary Cultures
|
|---|---|
| Chapter number | 2 |
| Book title |
Sertoli Cells
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7698-0_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7697-3, 978-1-4939-7698-0
|
| Authors |
Raquel L. Bernardino, Marco G. Alves, Pedro F. Oliveira, Bernardino, Raquel L., Alves, Marco G., Oliveira, Pedro F. |
| Abstract |
The purity of the primary cultures of Sertoli cells is a factor for the validation of the studies using this methodological approach. There is a probability of contamination of these cultures with other testicular cellular types, such as peritubular cells and germ cells (that represent a large percentage of the volume of the seminiferous tubules).For the evaluation of the purity of cultures, the immunocytochemistry technique (immunoperoxidase or immunofluorescence) is frequently used to label a protein marker specific for the Sertoli cells within the testicular environment. The expression of several protein markers can be used, with different particularities, being that vimentin is often used as a marker for Sertoli cells. Vimentin is expressed independently of the differentiation state of the cells and is observed around the nuclei and in the cytoplasm of Sertoli cells. |
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|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 33% |
| Student > Ph. D. Student | 2 | 17% |
| Student > Bachelor | 1 | 8% |
| Unspecified | 1 | 8% |
| Student > Master | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 2 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 8% |
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| Agricultural and Biological Sciences | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 6 | 50% |