Chapter title |
Profiling Signaling Proteins in Sertoli Cells by Co-immunoprecipitation
|
---|---|
Chapter number | 7 |
Book title |
Sertoli Cells
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7698-0_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7697-3, 978-1-4939-7698-0
|
Authors |
Maria João Freitas, Margarida Fardilha, Freitas, Maria João, Fardilha, Margarida |
Abstract |
Sertoli cells are crucial for germ cell support, create a suitable environment for spermatogenesis, and integrate information from the nervous system and germ cell line. To fully understand the role of Sertoli cells, it is necessary to characterize the protein-protein interactions. Identifying the interactome of a given protein may provide leads about the role and molecular mechanism of such protein in Sertoli cells. One of the techniques to characterize protein interactomes consists of co-immunoprecipitation followed by mass spectrometry or Western blot. Co-immunoprecipitation enables the isolation of a protein target and interactome under physiological conditions. In this chapter, we described how to perform an interactomic study using the co-immunoprecipitation technique in Sertoli cells. Moreover, we will focus on how to analyze and interpret the results of a co-immunoprecipitation before mass spectrometry analysis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 6% |
Student > Bachelor | 1 | 6% |
Unknown | 16 | 89% |
Readers by discipline | Count | As % |
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Unknown | 16 | 89% |