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Sertoli Cells

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Cover of 'Sertoli Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Establishment of Primary Culture of Sertoli Cells
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    Chapter 2 Evaluation of the Purity of Sertoli Cell Primary Cultures
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    Chapter 3 Preparation of Testicular Samples for Histology and Immunohistochemistry
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    Chapter 4 Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age
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    Chapter 5 Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy
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    Chapter 6 Sertoli Cell Preparation for Co-immunoprecipitation
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    Chapter 7 Profiling Signaling Proteins in Sertoli Cells by Co-immunoprecipitation
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    Chapter 8 Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy
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    Chapter 9 A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells
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    Chapter 10 Assessing Autophagy in Sertoli Cells
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    Chapter 11 Molecular Mechanisms and Signaling Pathways Involved in the Nutritional Support of Spermatogenesis by Sertoli Cells
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    Chapter 12 Assessing Sertoli Cell Metabolic Activity
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    Chapter 13 Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy
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    Chapter 14 Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs
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    Chapter 15 Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice
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    Chapter 16 Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of Actin-, and Microtubule-Based Cytoskeletons
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    Chapter 17 Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay
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    Chapter 18 Computational Methods Involved in Evaluating the Toxicity of the Reproductive Toxicants in Sertoli Cell
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    Chapter 19 A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells
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    Chapter 20 Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing
Attention for Chapter 14: Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs
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Chapter title
Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs
Chapter number 14
Book title
Sertoli Cells
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7698-0_14
Pubmed ID
Book ISBNs
978-1-4939-7697-3, 978-1-4939-7698-0
Authors

Hong Wang, Qingqing Yuan, Minghui Niu, Liping Wen, Hongyong Fu, Fan Zhou, Weihui Zhang, Zuping He, Wang, Hong, Yuan, Qingqing, Niu, Minghui, Wen, Liping, Fu, Hongyong, Zhou, Fan, Zhang, Weihui, He, Zuping

Abstract

Sertoli cells, as the unique somatic cells within the seminiferous tubules, play essential roles in regulating normal spermatogenesis. In addition, recent studies have demonstrated that Sertoli cells could have significant applications in regenerative medicine due to their great plasticity. However, the roles of genes in controlling the fate determinations of human Sertoli cells remain largely unknown. Silencing genes of human Sertoli cells utilizing small interfering RNAs (siRNAs) is an important method to explore their functions and mechanisms in human Sertoli cells. We isolated and identified human Sertoli cells. RNA interference (RNAi) was employed to probe the roles and signaling pathways of BMP6 and BMP4 in mediating the proliferation and apoptosis of human Sertoli cells. Specifically, siRNAs against BMP6 and BMP4 were used to knock down the expression levels of BMP6 and BMP4 and examine the function and mechanism in controlling the fate decisions of human Sertoli cells. In this chapter, we provided the detailed methods of RNAi in silencing BMP6 gene of human Sertoli cells. Quantitative real-time PCR demonstrated that the designed BMP6 siRNAs apparently silenced BMP6 mRNA in human Sertoli cells at 24 h after transfection. Western blots showed that the siRNAs silenced the expression of BMP6 protein effectively at 48 h after transfection. In summary, siRNAs can effectively and specifically knock down targeting genes at both transcriptional and translational levels utilizing RNAi in human Sertoli cells.

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Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 100%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 50%
Unknown 1 50%