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Mendeley readers
| Chapter title |
Detection of HPV E6/E7 mRNA in Clinical Samples Using RNA In Situ Hybridization
|
|---|---|
| Chapter number | 15 |
| Book title |
The Retinoblastoma Protein
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7565-5_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7564-8, 978-1-4939-7565-5
|
| Authors |
Manishkumar Pandey, Priyanka G. Bhosale, Manoj B. Mahimkar |
| Abstract |
Detection of human papilloma virus (HPV) in tissue specimens has been a clinical challenge since last 2 decades; however, screening for presence of E6/E7 transcripts is regarded as the gold standard, and it verifies the active HPV infection. Here, we describe "RNAscope®assay" a novel RNA in situ hybridization (ISH) technology; which detects E6/E7 mRNA of seven high risk HPV subtypes (HPV 16, 18, 31, 33, 35, 52, and 58) in formalin fixed paraffin embedded (FFPE) tissue samples. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 17% |
| Lecturer | 1 | 8% |
| Student > Doctoral Student | 1 | 8% |
| Other | 1 | 8% |
| Professor | 1 | 8% |
| Other | 3 | 25% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 3 | 25% |
| Biochemistry, Genetics and Molecular Biology | 2 | 17% |
| Chemistry | 1 | 8% |
| Unknown | 6 | 50% |