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The Retinoblastoma Protein

Overview of attention for book
Cover of 'The Retinoblastoma Protein'

Table of Contents

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    Book Overview
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    Chapter 1 Characterization of RB1 Deletions in Interphase and Metaphase by Molecular Cytogenetics Exemplified in Chronic Lymphatic Leukemia
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    Chapter 2 Detection of RB1 Gene Copy Number Variations Using a Multiplex Ligation-Dependent Probe Amplification Method
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    Chapter 3 A Fluorescent Quantitative Multiplex PCR Method to Detect Copy Number Changes in the RB1 Gene
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    Chapter 4 Using Methylation-Specific PCR to Study RB1 Promoter Hypermethylation
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    Chapter 5 Detection of Aberrant DNA Methylation Patterns in the RB1 Gene
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    Chapter 6 Detection of Retinoblastoma Protein Phosphorylation by Immunoblot Analysis
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    Chapter 7 Immunohistochemical Detection of the Retinoblastoma Protein
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    Chapter 8 Immunohistochemical Detection of Retinoblastoma Protein Phosphorylation in Human Tumor Samples
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    Chapter 9 Detection of CCND1 Locus Amplification by Fluorescence In Situ Hybridization
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    Chapter 10 Detection of CCND1 Gene Copy Number Variations Using Multiplex Ligation-Dependent Probe Amplification and Fluorescence In Situ Hybridization Methods
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    Chapter 11 Detection of p16 Promoter Hypermethylation by Methylation-Specific PCR
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    Chapter 12 Immunohistochemical Detection of p16 in Clinical Samples
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    Chapter 13 Detection of E2F-DNA Complexes Using Chromatin Immunoprecipitation Assays
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    Chapter 14 Detection of E2F-Induced Transcriptional Activity Using a Dual Luciferase Reporter Assay
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    Chapter 15 Detection of HPV E6/E7 mRNA in Clinical Samples Using RNA In Situ Hybridization
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    Chapter 16 CRISPR/Cas9-Mediated Knockout of Rb1 in Xenopus tropicalis
Attention for Chapter 4: Using Methylation-Specific PCR to Study RB1 Promoter Hypermethylation
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Chapter title
Using Methylation-Specific PCR to Study RB1 Promoter Hypermethylation
Chapter number 4
Book title
The Retinoblastoma Protein
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7565-5_4
Pubmed ID
29468541
Book ISBNs
978-1-4939-7564-8, 978-1-4939-7565-5
Authors

Thaís M. McCormick, Maria Da Glória Da C. Carvalho

Abstract

It has increasingly been considered crucial the understanding of DNA methylation of Tumor Suppressor Gene (TSG) promoters, such as that of retinoblastoma 1 gene (RB1), and its role during carcinogenesis. We present a detailed and optimized protocol of the methylation-specific PCR (MSP) technique to study RB1 gene promoter hypermethylation.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 75%
Unknown 1 25%
Readers by discipline Count As %
Medicine and Dentistry 2 50%
Nursing and Health Professions 1 25%
Unknown 1 25%

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