Chapter title |
Analysis of Muscle Stem Cell Fate Through Modulation of AMPK Activity
|
---|---|
Chapter number | 34 |
Book title |
AMPK
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7598-3_34 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7597-6, 978-1-4939-7598-3
|
Authors |
Marine Theret, Linda Gsaier, Sabrina Ben Larbi, Michèle Weiss-Gayet, Rémi Mounier |
Abstract |
In this chapter, we describe the methods to isolate and culture muscle stem cells (MuSCs) from murine skeletal muscle in order to decipher the intrinsic effect of AMP-activated kinase activity on MuSC fate. Culture of MuSCs is a powerful model to recapitulate every step of stem cell behavior observed in vivo: activation, proliferation, differentiation, fusion and also self-renewal. We provide the detailed procedures to isolate pure MuSCs by a flow cytometry-based method using the selection of a combination of specific markers and to characterize MuSC fate (quiescence, activation, and differentiation) in response to AMPK activity modulation by assessing of the expression of stem cell (e.g., Pax7) and myogenic marker (e.g., MyoD). |
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