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Characterization of a novel breast cancer cell line derived from a metastatic bone lesion of a breast cancer patient

Overview of attention for article published in Breast Cancer Research and Treatment, February 2018
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (63rd percentile)
  • Good Attention Score compared to outputs of the same age and source (65th percentile)

Mentioned by

twitter
5 tweeters

Readers on

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15 Mendeley
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Title
Characterization of a novel breast cancer cell line derived from a metastatic bone lesion of a breast cancer patient
Published in
Breast Cancer Research and Treatment, February 2018
DOI 10.1007/s10549-018-4719-9
Pubmed ID
Authors

Julie Johnson, Darrell C. Bessette, Jodi M. Saunus, Chanel E. Smart, Sarah Song, Rebecca L. Johnston, Sibylle Cocciardi, Esdy N. Rozali, Cameron N. Johnstone, Ana Christina Vargas, Stephen H. Kazakoff, Victorian Cancer BioBank, Kum Kum Khanna, Sunil R. Lakhani, Georgia Chenevix-Trench, Peter T. Simpson, Katia Nones, Nicola Waddell, Fares Al-Ejeh

Abstract

We aimed to generate and characterize a novel cell line from a breast cancer bone metastasis to better study the progression of the disease. The cell line, P7731, was derived from a metastatic bone lesion of a breast cancer patient and assessed for marker expression. P7731 was analyzed for DNA copy number variation, somatic mutations, and gene expression and was compared with the primary tumor. P7731 cells are negative for estrogen receptor alpha (ERα), progesterone receptor (PR), and HER2 (triple-negative); strongly express vimentin (100% of cells positive) and also express cytokeratins 8/18 and 19 but at lower frequencies. Flow cytometry indicates P7731 cells are predominantly CD44+/CD49f+/EpCAM-, consistent with a primitive, mesenchymal-like phenotype. The cell line is tumorigenic in immunocompromised mice. Exome sequencing identified a total of 45 and 76 somatic mutations in the primary tumor and cell line, respectively, of which 32 were identified in both samples and included mutations in known driver genes PIK3CA, TP53, and ARID1A. P7731 retains the DNA copy number alterations present in the matching primary tumor. Homozygous deletions detected in the cell line and in the primary tumor were found in regions containing three known (CDKN2A, CDKN2B, and CDKN1B) and 23 putative tumor suppressor genes. Cell line-specific gene amplification coupled with mRNA expression analysis revealed genes and pathways with potential pro-metastatic functions. This novel human breast cancer-bone metastasis cell line will be a useful model to study aspects of breast cancer biology, particularly metastasis-related changes from breast to bone.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 33%
Other 1 7%
Librarian 1 7%
Student > Doctoral Student 1 7%
Student > Master 1 7%
Other 1 7%
Unknown 5 33%
Readers by discipline Count As %
Medicine and Dentistry 4 27%
Biochemistry, Genetics and Molecular Biology 2 13%
Computer Science 1 7%
Agricultural and Biological Sciences 1 7%
Engineering 1 7%
Other 0 0%
Unknown 6 40%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 February 2020.
All research outputs
#4,148,982
of 14,539,453 outputs
Outputs from Breast Cancer Research and Treatment
#939
of 3,426 outputs
Outputs of similar age
#98,241
of 275,366 outputs
Outputs of similar age from Breast Cancer Research and Treatment
#21
of 60 outputs
Altmetric has tracked 14,539,453 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 3,426 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.3. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 275,366 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.
We're also able to compare this research output to 60 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.