↓ Skip to main content

Neurospora crassa developmental control mediated by the FLB-3 transcription factor

Overview of attention for article published in Fungal Biology, March 2018
Altmetric Badge

Mentioned by

twitter
4 X users

Citations

dimensions_citation
14 Dimensions

Readers on

mendeley
26 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Neurospora crassa developmental control mediated by the FLB-3 transcription factor
Published in
Fungal Biology, March 2018
DOI 10.1016/j.funbio.2018.01.004
Pubmed ID
Authors

Ana Carolina Boni, Daniela Luz Ambrósio, Fernanda Barbosa Cupertino, Alejandro Montenegro-Montero, Stela Virgilio, Fernanda Zanolli Freitas, Flávia Adolfo Corrocher, Rodrigo Duarte Gonçalves, Ally Yang, Matthew T. Weirauch, Timothy R. Hughes, Luis F. Larrondo, Maria Célia Bertolini

Abstract

Here, we report that the Neurospora crassa FLB-3 protein, the ortholog of the Aspergillus nidulans FlbC transcription factor, is required for developmental control. Deletion of flb-3 leads to changes in hyphae morphology and affects sexual and asexual development. We identified, as putative FLB-3 targets, the N. crassa aba-1, wet-1 and vos-1 genes, orthologs of the ones involved in A. nidulans asexual development and that work downstream of FlbC (abaA, wetA and vosA). In N. crassa, these three genes require FLB-3 for proper expression; however, they appear not to be required for normal development, as demonstrated by gene expression analyses during vegetative growth and asexual development. Moreover, mutant strains in the three genes conidiate well and produce viable conidia. We also determined FLB-3 DNA-binding preferences via protein-binding microarrays (PBMs) and demonstrated by chromatin immunoprecipitation (ChIP) that FLB-3 binds the aba-1, wet-1 and vos-1 promoters. Our data support an important role for FLB-3 in N. crassa development and highlight differences between the regulatory pathways controlled by this transcription factor in different fungal species.

X Demographics

X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 12%
Other 3 12%
Student > Ph. D. Student 3 12%
Student > Bachelor 2 8%
Professor > Associate Professor 2 8%
Other 5 19%
Unknown 8 31%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 27%
Agricultural and Biological Sciences 5 19%
Environmental Science 1 4%
Immunology and Microbiology 1 4%
Energy 1 4%
Other 1 4%
Unknown 10 38%