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Critical Function of AP-2gamma/TCFAP2C in Mouse Embryonic Germ Cell Maintenance1

Overview of attention for article published in Biology of Reproduction, January 2010
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  • Good Attention Score compared to outputs of the same age (67th percentile)
  • High Attention Score compared to outputs of the same age and source (87th percentile)

Mentioned by

wikipedia
1 Wikipedia page

Citations

dimensions_citation
111 Dimensions

Readers on

mendeley
109 Mendeley
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Title
Critical Function of AP-2gamma/TCFAP2C in Mouse Embryonic Germ Cell Maintenance1
Published in
Biology of Reproduction, January 2010
DOI 10.1095/biolreprod.109.078717
Pubmed ID
Authors

Susanne Weber, Dawid Eckert, Daniel Nettersheim, Ad J.M. Gillis, Sabine Schäfer, Peter Kuckenberg, Julia Ehlermann, Uwe Werling, Katharina Biermann, Leendert H.J. Looijenga, Hubert Schorle

Abstract

Formation of the germ cell lineage involves multiple processes, including repression of somatic differentiation and reacquisition of pluripotency as well as a unique epigenetic constitution. The transcriptional regulator Prdm1 has been identified as a main coordinator of this process, controlling epigenetic modification and gene expression. Here we report on the expression pattern of the transcription factor Tcfap2c, a putative downstream target of Prdm1, during normal mouse embryogenesis and the consequences of its specific loss in primordial germ cells (PGCs) and their derivatives. Tcfap2c is expressed in PGCs from Embryonic Day 7.25 (E 7.25) up to E 12.5, and targeted disruption resulted in sterile animals, both male and female. In the mutant animals, PGCs were specified but were lost around E 8.0. PGCs generated in vitro from embryonic stem cells lacking TCFAP2C displayed induction of Prdm1 and Dppa3. Upregulation of Hoxa1, Hoxb1, and T together with lack of expression of germ cell markers such Nanos3, Dazl, and Mutyh suggested that the somatic gene program is induced in TCFAP2C-deficient PGCs. Repression of TCFAP2C in TCam-2, a human PGC-resembling seminoma cell line, resulted in specific upregulation of HOXA1, HOXB1, MYOD1, and HAND1, indicative of mesodermal differentiation. Expression of genes indicative of ectodermal, endodermal, or extraembryonic differentiation, as well as the finding of no change to epigenetic modifications, suggested control by other factors. Our results implicate Tcfap2c as an important effector of Prdm1 activity that is required for PGC maintenance, most likely mediating Prdm1-induced suppression of mesodermal differentiation.

Mendeley readers

The data shown below were compiled from readership statistics for 109 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 <1%
Germany 1 <1%
France 1 <1%
United Kingdom 1 <1%
United States 1 <1%
Unknown 104 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 36 33%
Researcher 22 20%
Student > Master 12 11%
Student > Bachelor 9 8%
Student > Doctoral Student 6 6%
Other 15 14%
Unknown 9 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 48 44%
Biochemistry, Genetics and Molecular Biology 38 35%
Medicine and Dentistry 7 6%
Chemistry 2 2%
Pharmacology, Toxicology and Pharmaceutical Science 2 2%
Other 2 2%
Unknown 10 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 April 2014.
All research outputs
#4,025,863
of 13,494,252 outputs
Outputs from Biology of Reproduction
#785
of 4,024 outputs
Outputs of similar age
#80,175
of 279,200 outputs
Outputs of similar age from Biology of Reproduction
#7
of 65 outputs
Altmetric has tracked 13,494,252 research outputs across all sources so far. This one is in the 49th percentile – i.e., 49% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,024 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,200 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 65 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.