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Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy

Overview of attention for article published in Memórias do Instituto Oswaldo Cruz, February 2018
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Title
Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy
Published in
Memórias do Instituto Oswaldo Cruz, February 2018
DOI 10.1590/0074-02760170467
Pubmed ID
Authors

Pedro Henrique Ferreira Marçal, Lúcia Alves de Oliveira Fraga, Ana Márcia Menezes de Mattos, Laura Menegati, Angélica da Conceição Oliveira Coelho, Roberta Olmo Pinheiro, Euzenir Nunes Sarno, Malcolm S Duthie, Henrique Couto Teixeira

Abstract

BACKGROUND Leprosy remains a health problem in many countries, with difficulties in diagnosis resulting in delayed treatment and more severe disabilities. Antibodies against several Mycobacterium leprae antigens have, however, shown value as diagnostic and/or prognostic markers. OBJECTIVES The objective of this study was to evaluate serum immunoglobulin (Ig) IgM and IgG subclass reactivity against three M. leprae specific antigens: NDO-HSA, a conjugate formed by natural octyl disaccharide bound to human serum albumin; LID-1, the fusion protein product of the ml0405 and ml2331 genes; and NDO-LID, a combination of LID-1 and NDO. METHODS Sera from healthy controls, paucibacillary (PB) and multibacillary (MB) leprosy patients, and their respective household contacts, were evaluated for the presence of antigen-specific IgM, IgG, and IgG subclass antibodies by enzyme-linked immunosorbent assay (ELISA). The sensitivity and specificity of each ELISA were evaluated by receiver operating characteristic (ROC) curve analysis. FINDINGS Our data confirm that serum IgM antibodies against NDO-HSA and IgG antibodies against LID-1, as well as IgG/M antibodies against NDO-LID, are markedly increased in MB patients. For the first time, our data reveal a selective increase in IgG1 and IgG3 antibodies against LID-1 and NDO-LID in MB patients, demonstrating that these antibody isotypes are suitable for differentiation between MB and PB patients. ROC curve analysis indicates an improved capacity for diagnosing MB leprosy patients using the detection of IgG antibodies, particularly the IgG1 isotype, specific to LID-1 and NDO-LID over the performance levels attained with NDO-HSA. CONCLUSIONS Our findings indicate that serological tests based on the detection of antigen-specific IgG1 antibodies are a useful tool to differentiate MB from PB patients, and indicate the enhanced performance of the LID-1 and NDO-LID antigens in the serodiagnosis of leprosy.

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Mendeley readers

The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 61 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 15%
Student > Master 9 15%
Student > Ph. D. Student 5 8%
Student > Doctoral Student 5 8%
Student > Bachelor 4 7%
Other 10 16%
Unknown 19 31%
Readers by discipline Count As %
Immunology and Microbiology 12 20%
Medicine and Dentistry 7 11%
Biochemistry, Genetics and Molecular Biology 5 8%
Nursing and Health Professions 5 8%
Agricultural and Biological Sciences 4 7%
Other 7 11%
Unknown 21 34%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 March 2018.
All research outputs
#14,034,097
of 15,915,455 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#970
of 1,137 outputs
Outputs of similar age
#242,691
of 280,497 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#13
of 17 outputs
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