Title |
Localization of deformed wing virus infection in queen and drone Apis mellifera L
|
---|---|
Published in |
Virology Journal, March 2006
|
DOI | 10.1186/1743-422x-3-16 |
Pubmed ID | |
Authors |
Julie Fievet, Diana Tentcheva, Laurent Gauthier, Joachim de Miranda, François Cousserans, Marc Edouard Colin, Max Bergoin |
Abstract |
The distribution of deformed wing virus infection within the honey bee reproductive castes (queens, drones) was investigated by in situ hybridization and immunohistology from paraffin embedded sections. Digoxygenin or CY5.5 fluorochrome end-labelled nucleotide probes hybridizing to the 3' portion of the DWV genome were used to identify DWV RNA, while a monospecific antibody to the DWV-VP1 structural protein was used to identify viral proteins and particles. The histological data were confirmed by quantitative RT-PCR of dissected organs. Results showed that DWV infection is not restricted to the digestive tract of the bee but spread in the whole body, including queen ovaries, queen fat body and drone seminal vesicles. |
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Geographical breakdown
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Mexico | 2 | 2% |
United States | 2 | 2% |
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Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 19 | 17% |
Researcher | 18 | 16% |
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Student > Bachelor | 7 | 6% |
Other | 19 | 17% |
Unknown | 19 | 17% |
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Veterinary Science and Veterinary Medicine | 4 | 4% |
Other | 10 | 9% |
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