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Exocytosis and Endocytosis

Overview of attention for book
Cover of 'Exocytosis and Endocytosis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Pharmacological Inhibitors of Exocytosis and Endocytosis: Novel Bullets for Old Targets
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    Chapter 2 Systematic Analysis of Endocytosis by Cellular Perturbations
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    Chapter 3 Real-Time Detection of SNARE Complex Assembly with FRET Using the Tetracysteine System
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    Chapter 4 Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptides
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    Chapter 5 Secretion of circular proteins using sortase.
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    Chapter 6 Fractionation of Subcellular Membrane Vesicles of Epithelial and Non-epithelial Cells by OptiPrep™ Density Gradient Ultracentrifugation
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    Chapter 7 Combining Pulsed SILAC Labeling and Click-Chemistry for Quantitative Secretome Analysis.
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    Chapter 8 Probabilistic Density Maps to Study the Spatial Organization of Endocytosis
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    Chapter 9 Use of Kaede and Kikume Green–Red Fusions for Live Cell Imaging of G Protein-Coupled Receptors
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    Chapter 10 HaloTag as a Tool to Investigate Peroxisome Dynamics in Cultured Mammalian Cells
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    Chapter 11 SNAP-Tag to Monitor Trafficking of Membrane Proteins in Polarized Epithelial Cells
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    Chapter 12 FlAsH-PALM: Super-resolution Pointillist Imaging with FlAsH-Tetracysteine Labeling
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    Chapter 13 Analysis of protein dynamics with tandem fluorescent protein timers.
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    Chapter 14 Synchronization of Secretory Cargos Trafficking in Populations of Cells
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    Chapter 15 Use of Transmembrane FRET to Investigate the Internalization of Glycosylated Proteins
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    Chapter 16 A Method to Rapidly Induce Organelle-Specific Molecular Activities and Membrane Tethering
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    Chapter 17 A Novel Pair of Split Venus Fragments to Detect Protein–Protein Interactions by In Vitro and In Vivo Bimolecular Fluorescence Complementation Assays
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    Chapter 18 Real-Time Investigation of Plasma Membrane Deformation and Fusion Pore Expansion Using Polarized Total Internal Reflection Fluorescence Microscopy
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    Chapter 19 Nanocones to Study Initial Steps of Endocytosis
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    Chapter 20 A Novel Permeabilization Protocol to Obtain Intracellular 3D Immunolabeling for Electron Tomography
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    Chapter 21 VIS2FIX: Rapid Chemical Fixation of Vitreous Sections for Immuno-Electron Microscopy
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    Chapter 22 Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using the Endomembrane Trafficking Network
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    Chapter 23 Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in C. elegans
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    Chapter 24 Visualization of clathrin-mediated endocytosis in live Drosophila egg chambers.
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    Chapter 25 A novel extraction protocol to probe the role of cholesterol in synaptic vesicle recycling.
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    Chapter 26 Microfluidic devices for imaging trafficking events in vivo using genetic model organisms.
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    Chapter 27 The “In Situ” Proximity Ligation Assay to Probe Protein–Protein Interactions in Intact Tissues
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    Chapter 28 Exocytosis and Endocytosis
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    Chapter 29 Measurement of Dynamic F-Actin Changes During Exocytosis
Attention for Chapter 24: Visualization of clathrin-mediated endocytosis in live Drosophila egg chambers.
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Chapter title
Visualization of clathrin-mediated endocytosis in live Drosophila egg chambers.
Chapter number 24
Book title
Exocytosis and Endocytosis
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-0944-5_24
Pubmed ID
Book ISBNs
978-1-4939-0943-8, 978-1-4939-0944-5

Anupma Jha, Linton M Traub, Linton M. Traub


In oviparous animals, clathrin-dependent endocytosis is often critical to stockpile a necessary supply of yolk within the maturing oocyte, which enables subsequent embryonic development. In the physically linked chains of maturing egg chambers within the Drosophila melanogaster ovary, a distinct, morphologically discernable subset undergoes a massive burst clathrin-mediated endocytosis to accumulate yolk in a process termed vitellogenesis. Here, we describe how to prepare isolated ovaries to follow endocytosis, and detail approaches to follow live uptake of soluble reporters into vitellogenic Drosophila egg chambers.

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Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 29%
Professor > Associate Professor 2 29%
Student > Postgraduate 2 29%
Unknown 1 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 43%
Biochemistry, Genetics and Molecular Biology 1 14%
Chemical Engineering 1 14%
Neuroscience 1 14%
Unknown 1 14%

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