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Chapter title |
Visualization of clathrin-mediated endocytosis in live Drosophila egg chambers.
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Chapter number | 24 |
Book title |
Exocytosis and Endocytosis
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Published in |
Methods in molecular biology, January 2014
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DOI | 10.1007/978-1-4939-0944-5_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0943-8, 978-1-4939-0944-5
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Authors |
Anupma Jha, Linton M Traub, Linton M. Traub, Jha, Anupma, Traub, Linton M. |
Abstract |
In oviparous animals, clathrin-dependent endocytosis is often critical to stockpile a necessary supply of yolk within the maturing oocyte, which enables subsequent embryonic development. In the physically linked chains of maturing egg chambers within the Drosophila melanogaster ovary, a distinct, morphologically discernable subset undergoes a massive burst clathrin-mediated endocytosis to accumulate yolk in a process termed vitellogenesis. Here, we describe how to prepare isolated ovaries to follow endocytosis, and detail approaches to follow live uptake of soluble reporters into vitellogenic Drosophila egg chambers. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 17% |
Professor | 2 | 17% |
Student > Postgraduate | 2 | 17% |
Professor > Associate Professor | 2 | 17% |
Lecturer | 1 | 8% |
Other | 0 | 0% |
Unknown | 3 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 25% |
Agricultural and Biological Sciences | 3 | 25% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Chemical Engineering | 1 | 8% |
Neuroscience | 1 | 8% |
Other | 0 | 0% |
Unknown | 3 | 25% |