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Exocytosis and Endocytosis

Overview of attention for book
Cover of 'Exocytosis and Endocytosis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Pharmacological Inhibitors of Exocytosis and Endocytosis: Novel Bullets for Old Targets
  3. Altmetric Badge
    Chapter 2 Systematic Analysis of Endocytosis by Cellular Perturbations
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    Chapter 3 Real-Time Detection of SNARE Complex Assembly with FRET Using the Tetracysteine System
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    Chapter 4 Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptides
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    Chapter 5 Secretion of circular proteins using sortase.
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    Chapter 6 Fractionation of Subcellular Membrane Vesicles of Epithelial and Non-epithelial Cells by OptiPrep™ Density Gradient Ultracentrifugation
  8. Altmetric Badge
    Chapter 7 Combining Pulsed SILAC Labeling and Click-Chemistry for Quantitative Secretome Analysis.
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    Chapter 8 Probabilistic Density Maps to Study the Spatial Organization of Endocytosis
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    Chapter 9 Use of Kaede and Kikume Green–Red Fusions for Live Cell Imaging of G Protein-Coupled Receptors
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    Chapter 10 HaloTag as a Tool to Investigate Peroxisome Dynamics in Cultured Mammalian Cells
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    Chapter 11 SNAP-Tag to Monitor Trafficking of Membrane Proteins in Polarized Epithelial Cells
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    Chapter 12 FlAsH-PALM: Super-resolution Pointillist Imaging with FlAsH-Tetracysteine Labeling
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    Chapter 13 Analysis of protein dynamics with tandem fluorescent protein timers.
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    Chapter 14 Synchronization of Secretory Cargos Trafficking in Populations of Cells
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    Chapter 15 Use of Transmembrane FRET to Investigate the Internalization of Glycosylated Proteins
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    Chapter 16 A Method to Rapidly Induce Organelle-Specific Molecular Activities and Membrane Tethering
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    Chapter 17 A Novel Pair of Split Venus Fragments to Detect Protein–Protein Interactions by In Vitro and In Vivo Bimolecular Fluorescence Complementation Assays
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    Chapter 18 Real-Time Investigation of Plasma Membrane Deformation and Fusion Pore Expansion Using Polarized Total Internal Reflection Fluorescence Microscopy
  20. Altmetric Badge
    Chapter 19 Nanocones to Study Initial Steps of Endocytosis
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    Chapter 20 A Novel Permeabilization Protocol to Obtain Intracellular 3D Immunolabeling for Electron Tomography
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    Chapter 21 VIS2FIX: Rapid Chemical Fixation of Vitreous Sections for Immuno-Electron Microscopy
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    Chapter 22 Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using the Endomembrane Trafficking Network
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    Chapter 23 Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in C. elegans
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    Chapter 24 Visualization of clathrin-mediated endocytosis in live Drosophila egg chambers.
  26. Altmetric Badge
    Chapter 25 A novel extraction protocol to probe the role of cholesterol in synaptic vesicle recycling.
  27. Altmetric Badge
    Chapter 26 Microfluidic devices for imaging trafficking events in vivo using genetic model organisms.
  28. Altmetric Badge
    Chapter 27 The “In Situ” Proximity Ligation Assay to Probe Protein–Protein Interactions in Intact Tissues
  29. Altmetric Badge
    Chapter 28 Exocytosis and Endocytosis
  30. Altmetric Badge
    Chapter 29 Measurement of Dynamic F-Actin Changes During Exocytosis
Attention for Chapter 7: Combining Pulsed SILAC Labeling and Click-Chemistry for Quantitative Secretome Analysis.
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Chapter title
Combining Pulsed SILAC Labeling and Click-Chemistry for Quantitative Secretome Analysis.
Chapter number 7
Book title
Exocytosis and Endocytosis
Published in
Methods in molecular biology, June 2014
DOI 10.1007/978-1-4939-0944-5_7
Pubmed ID
Book ISBNs
978-1-4939-0943-8, 978-1-4939-0944-5
Authors

Eichelbaum K, Krijgsveld J, Katrin Eichelbaum, Jeroen Krijgsveld

Abstract

Secreted proteins, such as cytokines, chemokines, and hormones, exhibit central functions in intercellular communication, which is crucial to maintain homeostasis in every multicellular organism. A common approach to identify secreted proteins is by proteomic analysis of culture media after conditioning with a cell type of interest. This is preferably done in serum-free conditions to enable the detection of low-abundance secretory factors that would otherwise be masked by serum proteins. However, serum starvation introduces the risk of bringing cells in a stressed or perturbed state. A superior approach employs the enrichment of newly synthesized and secreted proteins from serum-containing growth medium. This is achieved by the combination of two metabolic labels: stable isotope-labeled amino acids for reliable quantification, and azidohomoalanine (AHA), an azide-bearing analogue of methionine, for the enrichment of newly synthesized and secreted proteins. This approach has been used to compare secretomes of multiple cell lines or to analyze proteins that are secreted upon a specific stimulation. Here we describe in detail the enrichment and quantification of newly synthesized and secreted proteins.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
Unknown 33 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 24%
Researcher 5 15%
Student > Master 5 15%
Student > Bachelor 3 9%
Unspecified 2 6%
Other 4 12%
Unknown 7 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 12 35%
Agricultural and Biological Sciences 7 21%
Unspecified 2 6%
Immunology and Microbiology 2 6%
Medicine and Dentistry 1 3%
Other 2 6%
Unknown 8 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2015.
All research outputs
#3,327,029
of 4,691,566 outputs
Outputs from Methods in molecular biology
#1,645
of 3,415 outputs
Outputs of similar age
#114,008
of 163,851 outputs
Outputs of similar age from Methods in molecular biology
#203
of 398 outputs
Altmetric has tracked 4,691,566 research outputs across all sources so far. This one is in the 16th percentile – i.e., 16% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,415 research outputs from this source. They receive a mean Attention Score of 1.4. This one is in the 34th percentile – i.e., 34% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 163,851 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 398 others from the same source and published within six weeks on either side of this one. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.