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Three-dimensional sub–100 nm resolution fluorescence microscopy of thick samples

Overview of attention for article published in Nature Methods, May 2008
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (96th percentile)
  • High Attention Score compared to outputs of the same age and source (82nd percentile)

Mentioned by

blogs
1 blog
twitter
1 X user
patent
40 patents
wikipedia
1 Wikipedia page

Citations

dimensions_citation
734 Dimensions

Readers on

mendeley
731 Mendeley
citeulike
7 CiteULike
connotea
1 Connotea
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Title
Three-dimensional sub–100 nm resolution fluorescence microscopy of thick samples
Published in
Nature Methods, May 2008
DOI 10.1038/nmeth.1211
Pubmed ID
Authors

Manuel F Juette, Travis J Gould, Mark D Lessard, Michael J Mlodzianoski, Bhupendra S Nagpure, Brian T Bennett, Samuel T Hess, Joerg Bewersdorf

Abstract

Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 731 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 19 3%
Germany 11 2%
United Kingdom 10 1%
France 8 1%
Netherlands 5 <1%
Switzerland 3 <1%
Serbia 2 <1%
Japan 2 <1%
Spain 2 <1%
Other 13 2%
Unknown 656 90%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 226 31%
Researcher 181 25%
Student > Master 61 8%
Professor > Associate Professor 38 5%
Professor 34 5%
Other 116 16%
Unknown 75 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 190 26%
Physics and Astronomy 185 25%
Biochemistry, Genetics and Molecular Biology 72 10%
Engineering 69 9%
Chemistry 69 9%
Other 54 7%
Unknown 92 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 27. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 July 2023.
All research outputs
#1,344,372
of 24,411,829 outputs
Outputs from Nature Methods
#1,647
of 5,180 outputs
Outputs of similar age
#2,874
of 81,044 outputs
Outputs of similar age from Nature Methods
#8
of 41 outputs
Altmetric has tracked 24,411,829 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,180 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 35.8. This one has gotten more attention than average, scoring higher than 68% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 81,044 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 41 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.