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Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus

Overview of attention for article published in Revista do Instituto de Medicina Tropical de São Paulo, March 2018
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Title
Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus
Published in
Revista do Instituto de Medicina Tropical de São Paulo, March 2018
DOI 10.1590/s1678-9946201860015
Pubmed ID
Authors

Maria Isabel de Oliveira, Gislene Mitsue Namiyama, Gabriela Bastos Cabral, João Leandro Ferreira, Noemi Taniwaki, Ana Maria Sardinha Afonso, Isabella Rillo Lima, Luís Fernando Macedo de Brigido

Abstract

A great variety of viruses which cause exanthema share other clinical manifestations, making the etiologic identification a very difficult task, relying exclusively on the clinical examination. Rubella virus (RV) infection during the early stages of pregnancy can lead to serious birth defects, known as congenital rubella syndrome (CRS). In the present report, we described the presence of Zika virus (ZIKV) particles in urine samples and also ZIKV isolation in SIRC cells from the urine of a patient in acute phase of suspected rubella disease. The 50-year-old unvaccinated woman living in Sao Paulo, Brazil, was admitted to the emergency room with fever, headache, rash, arthralgia and prostration. Urine samples were collected for virus isolation and RT-qPCR. SIRC and Vero cells were inoculated with urine samples during 7 days. RT-qPCR was performed using measles virus (MV) and RV primers and both were found to be negative. After this result, RT-qPCR was performed for parvovirus B19, herpes virus 6 and ZIKV. The urine sample and the isolate were positive by Real Time PCR for ZIKV and negative for all other viruses tested. The sequences isolated are from the Asiatic lineage.

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Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 37 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 19%
Researcher 7 19%
Student > Doctoral Student 5 14%
Student > Bachelor 3 8%
Other 2 5%
Other 5 14%
Unknown 8 22%
Readers by discipline Count As %
Medicine and Dentistry 7 19%
Biochemistry, Genetics and Molecular Biology 6 16%
Immunology and Microbiology 3 8%
Social Sciences 2 5%
Arts and Humanities 2 5%
Other 5 14%
Unknown 12 32%