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Assaying sensory ciliopathies using calcium biosensor expression in zebrafish ciliated olfactory neurons

Overview of attention for article published in Cilia, March 2018
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (56th percentile)

Mentioned by

twitter
5 tweeters

Citations

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4 Dimensions

Readers on

mendeley
16 Mendeley
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Title
Assaying sensory ciliopathies using calcium biosensor expression in zebrafish ciliated olfactory neurons
Published in
Cilia, March 2018
DOI 10.1186/s13630-018-0056-1
Pubmed ID
Authors

Judith G. M. Bergboer, Cameron Wyatt, Christina Austin-Tse, Emre Yaksi, Iain A. Drummond

Abstract

Primary cilia mediate signal transduction by acting as an organizing scaffold for receptors, signalling proteins and ion channels. Ciliated olfactory sensory neurons (OSNs) organize olfactory receptors and ion channels on cilia and generate a calcium influx as a primary signal in odourant detection. In the zebrafish olfactory placode, ciliated OSNs and microvillus OSNs constitute the major OSN cell types with distinct odourant sensitivity. Using transgenic expression of the calcium biosensor GCaMP5 in OSNs, we analysed sensory cilia-dependent odour responses in live zebrafish, at individual cell resolution.oval/ift88mutant andift172knockdown zebrafish were compared with wild-type siblings to establish ciliated OSN sensitivity to different classes of odourants. oval/ift88 mutant andift172knockdown zebrafish showed fewer and severely shortened OSN cilia without a reduction in OSN number. The fraction of responding OSNs and response amplitudes to bile acids and food odour, both sensed by ciliated OSNs, were significantly reduced inift88mutants andift172-deficient embryos, while the amino acids responses were not significantly changed. Our approach presents a quantitative model for studying sensory cilia signalling using zebrafish OSNs. Our results also implicateift172-deficiency as a novel cause of hyposmia, a reduced sense of smell, highlighting the value of directly assaying sensory cilia signalling in vivo and supporting the idea that hyposmia can be used as a diagnostic indicator of ciliopathies.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 31%
Student > Ph. D. Student 4 25%
Student > Master 3 19%
Professor > Associate Professor 2 13%
Other 1 6%
Other 1 6%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 38%
Agricultural and Biological Sciences 4 25%
Unspecified 3 19%
Neuroscience 3 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 April 2018.
All research outputs
#6,721,814
of 12,781,938 outputs
Outputs from Cilia
#35
of 81 outputs
Outputs of similar age
#117,228
of 273,807 outputs
Outputs of similar age from Cilia
#2
of 2 outputs
Altmetric has tracked 12,781,938 research outputs across all sources so far. This one is in the 46th percentile – i.e., 46% of other outputs scored the same or lower than it.
So far Altmetric has tracked 81 research outputs from this source. They receive a mean Attention Score of 3.0. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 273,807 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one.