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DNA Methylation Is the Primary Silencing Mechanism for a Set of Germ Line- and Tumor-Specific Genes with a CpG-Rich Promoter

Overview of attention for article published in Molecular & Cellular Biology, November 1999
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

Mentioned by

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7 patents
wikipedia
1 Wikipedia page

Citations

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428 Dimensions

Readers on

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174 Mendeley
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Title
DNA Methylation Is the Primary Silencing Mechanism for a Set of Germ Line- and Tumor-Specific Genes with a CpG-Rich Promoter
Published in
Molecular & Cellular Biology, November 1999
DOI 10.1128/mcb.19.11.7327
Pubmed ID
Authors

Charles De Smet, Christophe Lurquin, Bernard Lethé, Valérie Martelange, Thierry Boon

Abstract

A subset of male germ line-specific genes, the MAGE-type genes, are activated in many human tumors, where they produce tumor-specific antigens recognized by cytolytic T lymphocytes. Previous studies on gene MAGE-A1 indicated that transcription factors regulating its expression are present in all tumor cell lines whether or not they express the gene. The analysis of two CpG sites located in the promoter showed a strong correlation between expression and demethylation. It was also shown that MAGE-A1 transcription was induced in cell cultures treated with demethylating agent 5'-aza-2'-deoxycytidine. We have now analyzed all of the CpG sites within the 5' region of MAGE-A1 and show that for all of them, demethylation correlates with the transcription of the gene. We also show that the induction of MAGE-A1 with 5'-aza-2'-deoxycytidine is stable and that in all the cell clones it correlates with demethylation, indicating that demethylation is necessary and sufficient to produce expression. Conversely, transfection experiments with in vitro-methylated MAGE-A1 sequences indicated that heavy methylation suffices to stably repress the gene in cells containing the transcription factors required for expression. Most MAGE-type genes were found to have promoters with a high CpG content. Remarkably, although CpG-rich promoters are classically unmethylated in all normal tissues, those of MAGE-A1 and LAGE-1 were highly methylated in somatic tissues. In contrast, they were largely unmethylated in male germ cells. We conclude that MAGE-type genes belong to a unique subset of germ line-specific genes that use DNA methylation as a primary silencing mechanism.

Mendeley readers

The data shown below were compiled from readership statistics for 174 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 2%
Spain 2 1%
Denmark 1 <1%
Canada 1 <1%
India 1 <1%
Chile 1 <1%
Hungary 1 <1%
Luxembourg 1 <1%
Croatia 1 <1%
Other 0 0%
Unknown 162 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 64 37%
Student > Bachelor 24 14%
Student > Master 21 12%
Researcher 17 10%
Professor 17 10%
Other 22 13%
Unknown 9 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 93 53%
Biochemistry, Genetics and Molecular Biology 34 20%
Medicine and Dentistry 14 8%
Immunology and Microbiology 7 4%
Neuroscience 2 1%
Other 9 5%
Unknown 15 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 May 2019.
All research outputs
#1,998,246
of 15,124,215 outputs
Outputs from Molecular & Cellular Biology
#332
of 10,015 outputs
Outputs of similar age
#41,576
of 287,806 outputs
Outputs of similar age from Molecular & Cellular Biology
#4
of 57 outputs
Altmetric has tracked 15,124,215 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 10,015 research outputs from this source. They receive a mean Attention Score of 4.2. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 287,806 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 57 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.