| Chapter title |
Cell Line Models of Molecular Subtypes of Colorectal Cancer
|
|---|---|
| Chapter number | 1 |
| Book title |
Colorectal Cancer
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7765-9_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7764-2, 978-1-4939-7765-9
|
| Authors |
Jennifer K. Mooi, Ian Y. Luk, John M. Mariadason, Mooi, Jennifer K., Luk, Ian Y., Mariadason, John M. |
| Abstract |
Colorectal cancer (CRC) is a genetically diverse disease necessitating the need for well-characterized and reproducible models to enable its accurate investigation. Recent genomic analyses have confirmed that CRC cell lines accurately retain the key genetic alterations and represent the major molecular subtypes of primary CRC, underscoring their value as powerful preclinical models. In this chapter we detail the important issues to consider when using CRC cell lines, the techniques used for their appropriate molecular classification, and the methods by which they are cultured in vitro and as subcutaneous xenografts in immune-compromised mice. A panel of commonly available CRC cell lines that have been characterized for key molecular subtypes is also provided as a resource for investigators to select appropriate models to address specific research questions. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 24% |
| Student > Master | 3 | 18% |
| Student > Bachelor | 1 | 6% |
| Student > Doctoral Student | 1 | 6% |
| Researcher | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 6 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 18% |
| Agricultural and Biological Sciences | 3 | 18% |
| Medicine and Dentistry | 2 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Unknown | 8 | 47% |