Chapter title |
Proteomic Profiling for Colorectal Cancer Biomarker Discovery
|
---|---|
Chapter number | 16 |
Book title |
Colorectal Cancer
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7765-9_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7764-2, 978-1-4939-7765-9
|
Authors |
Paula Álvarez-Chaver, Loretta De Chiara, Vicenta Soledad Martínez-Zorzano |
Abstract |
Nowadays, the ideal biomarker for colorectal cancer (CRC) has not been found. Two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) are suitable techniques for searching new biomarkers. In this chapter, we describe methodology for biomarker discovery based on a proteomic approach. In addition, special attention is given to the sample preparation, including protein extraction, fractionation, and cleanup, as we consider this a critical step. Comparing the proteomic profile of tumor and mucosa, we identified the nucleoside diphosphate kinase A (NDKA) protein as a candidate biomarker for CRC. Finally, we validated NDKA with an ELISA kit using serum samples from individuals of a screening cohort. Our results suggest that serum NDKA is a potential biomarker for screening of CRC and premalignant advanced adenomas (AA). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 35% |
Student > Master | 3 | 18% |
Student > Bachelor | 2 | 12% |
Other | 1 | 6% |
Lecturer | 1 | 6% |
Other | 1 | 6% |
Unknown | 3 | 18% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 7 | 41% |
Medicine and Dentistry | 4 | 24% |
Chemistry | 1 | 6% |
Chemical Engineering | 1 | 6% |
Unknown | 4 | 24% |