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Colorectal Cancer

Overview of attention for book
Cover of 'Colorectal Cancer'

Table of Contents

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    Book Overview
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    Chapter 1 Cell Line Models of Molecular Subtypes of Colorectal Cancer
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    Chapter 2 Dissecting Oncogenic RTK Pathways in Colorectal Cancer Initiation and Progression
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    Chapter 3 Identification of Response Elements on Promoters Using Site-Directed Mutagenesis and Chromatin Immunoprecipitation
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    Chapter 4 Identification and Functional Analysis of Gene Regulatory Sequences Interacting with Colorectal Tumor Suppressors
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    Chapter 5 Methods for In Vivo Functional Studies of Chromatin-Modifying Enzymes in Early Steps of Colon Carcinogenesis
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    Chapter 6 The Colorectal Cancer Microenvironment: Strategies for Studying the Role of Cancer-Associated Fibroblasts
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    Chapter 7 Methods for Assessing Apoptosis and Anoikis in Normal Intestine/Colon and Colorectal Cancer
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    Chapter 8 Molecular Analysis of the Microbiome in Colorectal Cancer
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    Chapter 9 Proteomics Analysis of Colorectal Cancer Cells
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    Chapter 10 Autophagic Flux Assessment in Colorectal Cancer Cells
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    Chapter 11 Classification of Colorectal Cancer in Molecular Subtypes by Immunohistochemistry
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    Chapter 12 Stool DNA Integrity Method for Colorectal Cancer Detection
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    Chapter 13 RT-qPCR for Fecal Mature MicroRNA Quantification and Validation
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    Chapter 14 A Stool Multitarget mRNA Assay for the Detection of Colorectal Neoplasms
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    Chapter 15 Colorectal Cancer Detection Using Targeted LC-MS Metabolic Profiling
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    Chapter 16 Proteomic Profiling for Colorectal Cancer Biomarker Discovery
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    Chapter 17 Tumor-Derived Microparticles to Monitor Colorectal Cancer Evolution
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    Chapter 18 Molecular Testing for the Treatment of Advanced Colorectal Cancer: An Overview
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    Chapter 19 Testing Cell-Based Immunotherapy for Colorectal Cancer
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    Chapter 20 Patient-Derived Xenograft Models of Colorectal Cancer: Procedures for Engraftment and Propagation
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    Chapter 21 Use of Organoids to Characterize Signaling Pathways in Cancer Initiation
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    Chapter 22 Identification of Novel Molecules Targeting Cancer Stem Cells
Attention for Chapter 14: A Stool Multitarget mRNA Assay for the Detection of Colorectal Neoplasms
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Chapter title
A Stool Multitarget mRNA Assay for the Detection of Colorectal Neoplasms
Chapter number 14
Book title
Colorectal Cancer
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7765-9_14
Pubmed ID
Book ISBNs
978-1-4939-7764-2, 978-1-4939-7765-9
Authors

Elizabeth Herring, Shigeru Kanaoka, Eric Tremblay, Jean-François Beaulieu

Abstract

Noninvasive screening methods for the detection of colorectal cancers (CRC) at curable stages rely on the identification of specific biomarkers. Our group has shown that mRNA stool assays represent a powerful and robust approach for the prediction of colorectal neoplasms. In this methodological chapter, we describe the procedures to isolate good quality stool RNA and the steps to evaluate the levels of specific host mRNA markers such as ITGA6, MYC, and GADD45B using TaqMan-based quantitative and droplet digital PCR approaches.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 14%
Lecturer 1 14%
Student > Master 1 14%
Unknown 4 57%
Readers by discipline Count As %
Medicine and Dentistry 2 29%
Engineering 1 14%
Unknown 4 57%