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Genomic Cloning and Promoter Analysis of Aortic Preferentially Expressed Gene-1 IDENTIFICATION OF A VASCULAR SMOOTH MUSCLE-SPECIFIC PROMOTER MEDIATED BY AN E BOX MOTIF*

Overview of attention for article published in Journal of Biological Chemistry, May 1999
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (71st percentile)
  • Above-average Attention Score compared to outputs of the same age and source (56th percentile)

Mentioned by

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1 patent
wikipedia
1 Wikipedia page

Citations

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19 Dimensions

Readers on

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4 Mendeley
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Title
Genomic Cloning and Promoter Analysis of Aortic Preferentially Expressed Gene-1 IDENTIFICATION OF A VASCULAR SMOOTH MUSCLE-SPECIFIC PROMOTER MEDIATED BY AN E BOX MOTIF*
Published in
Journal of Biological Chemistry, May 1999
DOI 10.1074/jbc.274.20.14344
Pubmed ID
Authors

Chung-Ming Hsieh, Shaw-Fang Yet, Matthew D. Layne, Masafumi Watanabe, Audrey M. Hong, Mark A. Perrella, Mu-En Lee

Abstract

Aortic preferentially expressed gene-1 (APEG-1) was originally identified as a 1.4-kilobase (kb) transcript preferentially expressed in differentiated vascular smooth muscle cells (VSMC). Its expression is markedly down-regulated in de-differentiated VSMC, suggesting a role for APEG-1 in VSMC differentiation. We have now determined that APEG-1 is a single-copy gene in the human, rat, and mouse genomes and have mapped human APEG-1 to chromosome 2q34. To study the molecular mechanisms regulating its expression, we characterized the genomic organization and promoter of mouse APEG-1. APEG-1 spans 4.5 kb in the mouse genome and is composed of five exons. Using reporter gene transfection analysis, we found that a 2. 7-kb APEG-1 5'-flanking sequence directed a high level of promoter activity only in VSMC. Its activity was minimal in five other cell types. A repressor region located within an upstream 685-base pair sequence suppressed the activity of this 2.7-kb promoter. Further deletion and mutation analyses identified an E box motif as a positive regulatory element, which was bound by nuclear protein prepared from VSMC. In conjunction with its flanking sequence, this E box motif confers VSMC-specific enhancer activity to a heterologous SV40 promoter. To our knowledge, this is the first demonstration of an E box motif that mediates gene expression restricted to VSMC.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 1 25%
Unknown 3 75%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 50%
Unknown 2 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2010.
All research outputs
#5,446,210
of 25,371,288 outputs
Outputs from Journal of Biological Chemistry
#13,967
of 85,238 outputs
Outputs of similar age
#5,593
of 36,586 outputs
Outputs of similar age from Journal of Biological Chemistry
#121
of 686 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 85,238 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 36,586 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 686 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.