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Protein Complex Assembly

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Cover of 'Protein Complex Assembly'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Experimental Characterization of Protein Complex Structure, Dynamics, and Assembly
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    Chapter 2 High-Throughput Electron Cryo-tomography of Protein Complexes and Their Assembly
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    Chapter 3 Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy
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    Chapter 4 Time-Resolved Cryo-electron Microscopy Using a Microfluidic Chip
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    Chapter 5 Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy
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    Chapter 6 Reconstitution of Isotopically Labeled Ribosomal Protein L29 in the 50S Large Ribosomal Subunit for Solution-State and Solid-State NMR
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    Chapter 7 Characterizing Protein-Protein Interactions Using Deep Sequencing Coupled to Yeast Surface Display
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    Chapter 8 Structurally Guided In Vivo Crosslinking
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    Chapter 9 Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry
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    Chapter 10 Hydrogen-Deuterium Exchange Mass Spectrometry to Study Protein Complexes
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    Chapter 11 Structural Analysis of Protein Complexes by Cross-Linking and Mass Spectrometry
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    Chapter 12 Global Characterization of Protein Complexes by Biochemical Purification-Mass Spectrometry (BP/MS)
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    Chapter 13 Proteomic Profiling of Integrin Adhesion Complex Assembly
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    Chapter 14 Dual-Color and 3D Super-Resolution Microscopy of Multi-protein Assemblies
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    Chapter 15 Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion
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    Chapter 16 Observing the Assembly of Protein Complexes in Living Eukaryotic Cells in Super-Resolution Using refSOFI
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    Chapter 17 Detecting Purinosome Metabolon Formation with Fluorescence Microscopy
  19. Altmetric Badge
    Chapter 18 Analysis of Bacterial Pilus Assembly by Shearing and Immunofluorescence Microscopy
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    Chapter 19 Expression, Purification, and Assembly of Archaellum Subcomplexes of Sulfolobus acidocaldarius
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    Chapter 20 Unstable Protein Purification Through the Formation of Stable Complexes
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    Chapter 21 Expressing Multi-subunit Complexes Using biGBac
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    Chapter 22 Computational Modelling of Protein Complex Structure and Assembly
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    Chapter 23 Inferring and Using Protein Quaternary Structure Information from Crystallographic Data
  25. Altmetric Badge
    Chapter 24 Searching and Extracting Data from the EMBL-EBI Complex Portal
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    Chapter 25 Automated Computational Inference of Multi-protein Assemblies from Biochemical Co-purification Data
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    Chapter 26 A Multiscale Computational Model for Simulating the Kinetics of Protein Complex Assembly
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    Chapter 27 Flexible Protein-Protein Docking with SwarmDock
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    Chapter 28 Protein-Protein Docking Using Evolutionary Information
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    Chapter 29 Modeling Structure and Dynamics of Protein Complexes with SAXS Profiles
  31. Altmetric Badge
    Chapter 30 Modeling the Structure of Helical Assemblies with Experimental Constraints in Rosetta
  32. Altmetric Badge
    Chapter 31 Selecting Conformational Ensembles Using Residual Electron and Anomalous Density (READ)
Attention for Chapter 25: Automated Computational Inference of Multi-protein Assemblies from Biochemical Co-purification Data
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Chapter title
Automated Computational Inference of Multi-protein Assemblies from Biochemical Co-purification Data
Chapter number 25
Book title
Protein Complex Assembly
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7759-8_25
Pubmed ID
Book ISBNs
978-1-4939-7758-1, 978-1-4939-7759-8
Authors

Florian Goebels, Lucas Hu, Gary Bader, Andrew Emili

Abstract

Biology has amassed a wealth of information about the function of a multitude of protein-coding genes across species. The challenge now is to understand how all these proteins work together to form a living organism, and a crucial step for gaining this knowledge is a complete description of the molecular "wiring circuits" that underlie cellular processes. In this chapter, we describe a general computational framework for predicting multi-protein assemblies from biochemical co-fractionation data.

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Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 38%
Student > Ph. D. Student 1 13%
Professor 1 13%
Student > Postgraduate 1 13%
Unknown 2 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 38%
Mathematics 1 13%
Computer Science 1 13%
Agricultural and Biological Sciences 1 13%
Unknown 2 25%

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