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Protein Complex Assembly

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Cover of 'Protein Complex Assembly'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Experimental Characterization of Protein Complex Structure, Dynamics, and Assembly
  3. Altmetric Badge
    Chapter 2 High-Throughput Electron Cryo-tomography of Protein Complexes and Their Assembly
  4. Altmetric Badge
    Chapter 3 Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy
  5. Altmetric Badge
    Chapter 4 Time-Resolved Cryo-electron Microscopy Using a Microfluidic Chip
  6. Altmetric Badge
    Chapter 5 Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy
  7. Altmetric Badge
    Chapter 6 Reconstitution of Isotopically Labeled Ribosomal Protein L29 in the 50S Large Ribosomal Subunit for Solution-State and Solid-State NMR
  8. Altmetric Badge
    Chapter 7 Characterizing Protein-Protein Interactions Using Deep Sequencing Coupled to Yeast Surface Display
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    Chapter 8 Structurally Guided In Vivo Crosslinking
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    Chapter 9 Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry
  11. Altmetric Badge
    Chapter 10 Hydrogen-Deuterium Exchange Mass Spectrometry to Study Protein Complexes
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    Chapter 11 Structural Analysis of Protein Complexes by Cross-Linking and Mass Spectrometry
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    Chapter 12 Global Characterization of Protein Complexes by Biochemical Purification-Mass Spectrometry (BP/MS)
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    Chapter 13 Proteomic Profiling of Integrin Adhesion Complex Assembly
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    Chapter 14 Dual-Color and 3D Super-Resolution Microscopy of Multi-protein Assemblies
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    Chapter 15 Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion
  17. Altmetric Badge
    Chapter 16 Observing the Assembly of Protein Complexes in Living Eukaryotic Cells in Super-Resolution Using refSOFI
  18. Altmetric Badge
    Chapter 17 Detecting Purinosome Metabolon Formation with Fluorescence Microscopy
  19. Altmetric Badge
    Chapter 18 Analysis of Bacterial Pilus Assembly by Shearing and Immunofluorescence Microscopy
  20. Altmetric Badge
    Chapter 19 Expression, Purification, and Assembly of Archaellum Subcomplexes of Sulfolobus acidocaldarius
  21. Altmetric Badge
    Chapter 20 Unstable Protein Purification Through the Formation of Stable Complexes
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    Chapter 21 Expressing Multi-subunit Complexes Using biGBac
  23. Altmetric Badge
    Chapter 22 Computational Modelling of Protein Complex Structure and Assembly
  24. Altmetric Badge
    Chapter 23 Inferring and Using Protein Quaternary Structure Information from Crystallographic Data
  25. Altmetric Badge
    Chapter 24 Searching and Extracting Data from the EMBL-EBI Complex Portal
  26. Altmetric Badge
    Chapter 25 Automated Computational Inference of Multi-protein Assemblies from Biochemical Co-purification Data
  27. Altmetric Badge
    Chapter 26 A Multiscale Computational Model for Simulating the Kinetics of Protein Complex Assembly
  28. Altmetric Badge
    Chapter 27 Flexible Protein-Protein Docking with SwarmDock
  29. Altmetric Badge
    Chapter 28 Protein-Protein Docking Using Evolutionary Information
  30. Altmetric Badge
    Chapter 29 Modeling Structure and Dynamics of Protein Complexes with SAXS Profiles
  31. Altmetric Badge
    Chapter 30 Modeling the Structure of Helical Assemblies with Experimental Constraints in Rosetta
  32. Altmetric Badge
    Chapter 31 Selecting Conformational Ensembles Using Residual Electron and Anomalous Density (READ)
Attention for Chapter 4: Time-Resolved Cryo-electron Microscopy Using a Microfluidic Chip
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Chapter title
Time-Resolved Cryo-electron Microscopy Using a Microfluidic Chip
Chapter number 4
Book title
Protein Complex Assembly
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7759-8_4
Pubmed ID
Book ISBNs
978-1-4939-7758-1, 978-1-4939-7759-8
Authors

Sandip Kaledhonkar, Ziao Fu, Howard White, Joachim Frank, Kaledhonkar, Sandip, Fu, Ziao, White, Howard, Frank, Joachim

Abstract

With the advent of direct electron detectors, cryo-EM has become a popular choice for molecular structure determination. Among its advantages over X-ray crystallography are (1) no need for crystals, (2) much smaller sample volumes, and (3) the ability to determine multiple structures or conformations coexisting in one sample. In principle, kinetic experiments can be done using standard cryo-EM, but mixing and freezing grids require several seconds. However, many biological processes are much faster than that time scale, and the ensuing short-lived states of the molecules cannot be captured. Here, we lay out a detailed protocol for how to capture such intermediate states on the millisecond time scale with time-resolved cryo-EM.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 52 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 15 29%
Researcher 9 17%
Student > Bachelor 4 8%
Student > Master 3 6%
Student > Postgraduate 3 6%
Other 2 4%
Unknown 16 31%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 24 46%
Physics and Astronomy 3 6%
Agricultural and Biological Sciences 3 6%
Mathematics 1 2%
Earth and Planetary Sciences 1 2%
Other 4 8%
Unknown 16 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 December 2018.
All research outputs
#18,594,219
of 23,031,582 outputs
Outputs from Methods in molecular biology
#7,974
of 13,177 outputs
Outputs of similar age
#330,599
of 442,391 outputs
Outputs of similar age from Methods in molecular biology
#950
of 1,499 outputs
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So far Altmetric has tracked 13,177 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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