Chapter title |
Structural Analysis of Protein Complexes by Cross-Linking and Mass Spectrometry
|
---|---|
Chapter number | 11 |
Book title |
Protein Complex Assembly
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7759-8_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7758-1, 978-1-4939-7759-8
|
Authors |
Moriya Slavin, Nir Kalisman |
Abstract |
Cross-linking and mass spectrometry is used more and more for the structural analysis of large proteins and protein complexes. Although essentially a low-resolution method, it avoids the main drawbacks of established structural techniques. Particularly, it is largely insensitive to the inherent flexibility of the studied complexes and is applied under native conditions. It is also applicable to nearly every structural system. Therefore, cross-linking and mass spectrometry is the method of choice for elucidating the general architecture of protein complexes. Advances in instrumentation, techniques, and software now allow every lab that is working with proteins to apply the approach without much difficulty. The most specialized step in the workflow, the mass spectrometry measurement, can be done in most facilities that are performing standard proteomics. We detail here a step-by-step protocol of how to successfully apply the approach in collaboration with the mass spectrometry facility in your institution. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 31 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 23% |
Student > Master | 6 | 19% |
Student > Bachelor | 3 | 10% |
Researcher | 3 | 10% |
Professor | 2 | 6% |
Other | 3 | 10% |
Unknown | 7 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 13 | 42% |
Agricultural and Biological Sciences | 3 | 10% |
Chemistry | 3 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
Medicine and Dentistry | 1 | 3% |
Other | 2 | 6% |
Unknown | 7 | 23% |