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CRISPR/Cas9-induced shank3b mutant zebrafish display autism-like behaviors

Overview of attention for article published in Molecular Autism, April 2018
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Title
CRISPR/Cas9-induced shank3b mutant zebrafish display autism-like behaviors
Published in
Molecular Autism, April 2018
DOI 10.1186/s13229-018-0204-x
Pubmed ID
Authors

Chun-xue Liu, Chun-yang Li, Chun-chun Hu, Yi Wang, Jia Lin, Yong-hui Jiang, Qiang Li, Xiu Xu

Abstract

Human genetic and genomic studies have supported a strong causal role ofSHANK3deficiency in autism spectrum disorder (ASD). However, the molecular mechanism underlyingSHANK3deficiency resulting in ASD is not fully understood. Recently, the zebrafish has become an attractive organism to model ASD because of its high efficiency of genetic manipulation and robust behavioral phenotypes. The orthologous gene to humanSHANK3is duplicated in the zebrafish genome and has two homologs,shank3aandshank3b. Previous studies have reportedshank3morphants in zebrafish using the morpholino method. Here, we report the generation and characterization ofshank3bmutant zebrafish in larval and adult stages using the CRISPR/Cas9 genome editing technique. CRISPR/Cas9 was applied to generate ashank3bloss-of-function mutation (shank3b -/- ) in zebrafish. A series of morphological measurements, behavioral tests, and molecular analyses were performed to systematically characterize the behavioral and molecular changes inshank3bmutant zebrafish. shank3b -/- zebrafish exhibited abnormal morphology in early development. They showed reduced locomotor activity both as larvae and adults, reduced social interaction and time spent near conspecifics, and significant repetitive swimming behaviors. Additionally, the levels of both postsynaptic homer1 and presynaptic synaptophysin were significantly reduced in the adult brain ofshank3b-deficient zebrafish. We generated the first inheritableshank3bmutant zebrafish model using CRISPR/Cas9 gene editing approach.shank3b-/-zebrafish displayed robust autism-like behaviors and altered levels of the synaptic proteins homer1 and synaptophysin. The versatility of zebrafish as a model for studying neurodevelopment and conducting drug screening will likely have a significant contribution to future studies of humanSHANK3function and ASD.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 195 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 195 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 29 15%
Student > Master 27 14%
Student > Bachelor 25 13%
Researcher 18 9%
Professor > Associate Professor 9 5%
Other 31 16%
Unknown 56 29%
Readers by discipline Count As %
Neuroscience 35 18%
Biochemistry, Genetics and Molecular Biology 31 16%
Agricultural and Biological Sciences 19 10%
Psychology 8 4%
Medicine and Dentistry 8 4%
Other 31 16%
Unknown 63 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 December 2022.
All research outputs
#13,695,105
of 23,931,222 outputs
Outputs from Molecular Autism
#534
of 690 outputs
Outputs of similar age
#162,973
of 332,330 outputs
Outputs of similar age from Molecular Autism
#17
of 22 outputs
Altmetric has tracked 23,931,222 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 690 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 28.5. This one is in the 21st percentile – i.e., 21% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 332,330 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.