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CpG Islands

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Cover of 'CpG Islands'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 CpG Islands: A Historical Perspective
  3. Altmetric Badge
    Chapter 2 Biochemical Identification of Nonmethylated DNA by BioCAP-Seq
  4. Altmetric Badge
    Chapter 3 Prediction of CpG Islands as an Intrinsic Clustering Property Found in Many Eukaryotic DNA Sequences and Its Relation to DNA Methylation
  5. Altmetric Badge
    Chapter 4 CpG Islands in Cancer: Heads, Tails, and Sides
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    Chapter 5 Infinium DNA Methylation Microarrays on Formalin-Fixed, Paraffin-Embedded Samples
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    Chapter 6 The Use of Methylation-Sensitive Multiplex Ligation-Dependent Probe Amplification for Quantification of Imprinted Methylation
  8. Altmetric Badge
    Chapter 7 The Pancancer DNA Methylation Trackhub: A Window to The Cancer Genome Atlas Epigenomics Data
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    Chapter 8 Methylation-Sensitive Amplification Length Polymorphism (MS-AFLP) Microarrays for Epigenetic Analysis of Human Genomes
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    Chapter 9 Genome-Wide Profiling of DNA Methyltransferases in Mammalian Cells
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    Chapter 10 Experimental Design and Bioinformatic Analysis of DNA Methylation Data
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    Chapter 11 Assay for Transposase Accessible Chromatin (ATAC-Seq) to Chart the Open Chromatin Landscape of Human Pancreatic Islets
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    Chapter 12 Defining Regulatory Elements in the Human Genome Using Nucleosome Occupancy and Methylome Sequencing (NOMe-Seq)
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    Chapter 13 Genome-Wide Mapping of Protein–DNA Interactions on Nascent Chromatin
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    Chapter 14 Analysis of Chromatin Interactions Mediated by Specific Architectural Proteins in Drosophila Cells
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    Chapter 15 High-Throughput Single-Cell RNA Sequencing and Data Analysis
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    Chapter 16 Functional Insulator Scanning of CpG Islands to Identify Regulatory Regions of Promoters Using CRISPR
  18. Altmetric Badge
    Chapter 17 An Application-Directed, Versatile DNA FISH Platform for Research and Diagnostics
Attention for Chapter 2: Biochemical Identification of Nonmethylated DNA by BioCAP-Seq
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Chapter title
Biochemical Identification of Nonmethylated DNA by BioCAP-Seq
Chapter number 2
Book title
CpG Islands
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7768-0_2
Pubmed ID
Book ISBNs
978-1-4939-7767-3, 978-1-4939-7768-0
Authors

Hannah K. Long, Nathan R. Rose, Neil P. Blackledge, Robert J. Klose

Abstract

CpG islands are regions of vertebrate genomes that often function as gene regulatory elements and are associated with most gene promoters. CpG island elements usually contain nonmethylated CpG dinucleotides, while the remainder of the genome is pervasively methylated. We developed a biochemical approach called biotinylated CxxC affinity purification (BioCAP) to unbiasedly isolate regions of the genome that contain nonmethylated CpG dinucleotides. The resulting highly pure nonmethylated DNA is easily analyzed by quantitative PCR to interrogate specific loci or via massively parallel sequencing to yield genome-wide profiles.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Professor 2 33%
Student > Ph. D. Student 2 33%
Other 1 17%
Unknown 1 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 67%
Chemistry 1 17%
Unknown 1 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 April 2018.
All research outputs
#10,180,707
of 12,745,229 outputs
Outputs from Methods in molecular biology
#4,301
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Outputs of similar age
#204,976
of 273,184 outputs
Outputs of similar age from Methods in molecular biology
#6
of 7 outputs
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