Glycosylation Engineering of Biopharmaceuticals

Chapter 1 Engineering of Therapeutic and Diagnostic O -Glycans on Recombinant Mucin-Type Immunoglobulin Fusion Proteins Expressed in CHO Cells

Chapter 2 Engineering a Human-Like Glycosylation to Produce Therapeutic Glycoproteins Based on 6-Linked Sialylation in CHO Cells

Chapter 3 Glycoengineered Pichia -Based Expression of Monoclonal Antibodies

Chapter 4 N-Glycosylation Humanization for Production of Therapeutic Recombinant Glycoproteins in Saccharomyces cerevisiae

Chapter 5 Engineering the baculovirus genome to produce galactosylated antibodies in lepidopteran cells.

Chapter 6 NanoLC Chips MS/MS for the Characterization of N -Glycopeptides Generated from Trypsin Digestion of a Monoclonal Antibody

Chapter 7 Cetuximab Fab and Fc N-Glycan Fast Characterization Using IdeS Digestion and Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry

Chapter 8 Therapeutic Antibody Glycosylation Analysis: A Contract Research Organization Perspective in the Frame of Batch Release or Comparability Support

Chapter 9 Mass Spectrometric Analysis of O -Linked Oligosaccharides from Various Recombinant Expression Systems

Chapter 10 Assessing Fc Glycan Heterogeneity of Therapeutic Recombinant Monoclonal Antibodies Using NP-HPLC

Chapter 11 Application of Capillary Electrophoresis in Glycoprotein Analysis

Chapter 12 Characterization of Glycoprotein Biopharmaceutical Products by Caliper LC90 CE-SDS Gel Technology

Chapter 13 Hydrophobic Interaction Chromatography to Analyze Glycoproteins

Chapter 14 Lectin Glycoprofiling of Recombinant Therapeutic Interleukin-7

Chapter 15 Analysis of Monoclonal Antibodies by Sedimentation Velocity Analytical Ultracentrifugation

Chapter 16 Noncovalent Mass Spectrometry for the Characterization of Antibody/Antigen Complexes

Chapter 17 Conformational Analysis of Recombinant Monoclonal Antibodies with Hydrogen/Deuterium Exchange Mass Spectrometry

Chapter 18 Epitope Mapping of Antibodies by Mass Spectroscopy: A Case Study

Chapter 19 Evaluation of Antibody-Dependent Cell Cytotoxicity Using Lactate Dehydrogenase (LDH) Measurement

Chapter 20 Evaluation of Complement-Dependent Cytotoxicity Using ATP Measurement and C1q/C4b Binding

Chapter 21 Capture of the Human IgG1 Antibodies by Protein A for the Kinetic Study of h-IgG/FcγR Interaction Using SPR-Based Biosensor Technology

Chapter 22 Mass Spectrometry Protocol for the Absolute Quantification of a Monoclonal Antibody in Serum with Immunopurification