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Arabidopsis Protocols

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Cover of 'Arabidopsis Protocols'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Handling Arabidopsis Plants: Growth, Preservation of Seeds, Transformation, and Genetic Crosses
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    Chapter 2 Using Arabidopsis-Related Model Species (ARMS): Growth, Genetic Transformation, and Comparative Genomics
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    Chapter 3 Growing Arabidopsis In Vitro: Cell Suspensions, In Vitro Culture, and Regeneration
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    Chapter 4 Arabidopsis Database and Stock Resources
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    Chapter 5 Bioinformatic Tools in Arabidopsis Research
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    Chapter 6 Exploiting Natural Variation in Arabidopsis
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    Chapter 7 Grafting in Arabidopsis.
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    Chapter 8 Agrobacterium tumefaciens-Mediated Transient Transformation of Arabidopsis thaliana Leaves
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    Chapter 9 iTILLING: Personalized Mutation Screening
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    Chapter 10 Tailor-Made Mutations in Arabidopsis Using Zinc Finger Nucleases
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    Chapter 11 Arabidopsis Protocols
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    Chapter 12 Generation and Identification of Arabidopsis EMS Mutants
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    Chapter 13 Generation and Characterization of Arabidopsis T-DNA Insertion Mutants
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    Chapter 14 Identification of EMS-Induced Causal Mutations in Arabidopsis thaliana by Next-Generation Sequencing.
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    Chapter 15 Arabidopsis Transformation with Large Bacterial Artificial Chromosomes
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    Chapter 16 Global DNA Methylation Analysis Using Methyl-Sensitive Amplification Polymorphism (MSAP)
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    Chapter 17 Next-Generation Mapping of Genetic Mutations Using Bulk Population Sequencing
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    Chapter 18 Chemical Fingerprinting of Arabidopsis Using Fourier Transform Infrared (FT-IR) Spectroscopic Approaches
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    Chapter 19 A Pipeline for (15)N Metabolic Labeling and Phosphoproteome Analysis in Arabidopsis thaliana.
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    Chapter 20 Gene expression profiling using DNA microarrays.
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    Chapter 21 Forward Chemical Genetic Screening
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    Chapter 22 Highly Reproducible ChIP-on-Chip Analysis to Identify Genome-Wide Protein Binding and Chromatin Status in Arabidopsis thaliana.
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    Chapter 23 Fluorescence Microscopy
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    Chapter 24 Immunocytochemical Fluorescent In Situ Visualization of Proteins In Arabidopsis
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    Chapter 25 High-pressure freezing and freeze substitution of Arabidopsis for electron microscopy.
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    Chapter 26 Applications of Fluorescent Marker Proteins in Plant Cell Biology
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    Chapter 27 Flow Cytometry and Sorting in Arabidopsis
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    Chapter 28 Live Imaging of Arabidopsis Development
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    Chapter 29 Arabidopsis organelle isolation and characterization.
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    Chapter 30 Analysis of Subcellular Metabolite Distributions Within Arabidopsis thaliana Leaf Tissue: A Primer for Subcellular Metabolomics.
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    Chapter 31 Hormone Profiling
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    Chapter 32 Purification of Protein Complexes and Characterization of Protein-Protein Interactions
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    Chapter 33 Protein Fragment Bimolecular Fluorescence Complementation Analyses for the In vivo Study of Protein-Protein Interactions and Cellular Protein Complex Localizations
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    Chapter 34 The Split-Ubiquitin System for the Analysis of Three-Component Interactions
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    Chapter 35 RNA-Binding Protein Immunoprecipitation from Whole-Cell Extracts
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    Chapter 36 High-Throughput Analysis of Protein-DNA Binding Affinity
Attention for Chapter 7: Grafting in Arabidopsis.
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Chapter title
Grafting in Arabidopsis.
Chapter number 7
Book title
Arabidopsis Protocols
Published in
Methods in molecular biology, August 2013
DOI 10.1007/978-1-62703-580-4_7
Pubmed ID
Book ISBNs
978-1-62703-579-8, 978-1-62703-580-4
Authors

Bainbridge K, Bennett T, Crisp P, Leyser O, Turnbull C, Bainbridge, Katherine, Bennett, Tom, Crisp, Peter, Leyser, Ottoline, Turnbull, Colin, Katherine Bainbridge, Tom Bennett, Peter Crisp, Ottoline Leyser, Colin Turnbull

Abstract

Grafting provides a simple way to generate chimeric plants with regions of different genotypes and thus to assess the cell autonomy of gene action. The technique of grafting has been widely used in other species, but in Arabidopsis, its small size makes the process rather more demanding. However, there are now several well-established grafting procedures available, which we described here, and their use has already contributed greatly to understanding of such processes as shoot branching control, flowering, disease resistance, and systemic silencing.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 19%
Researcher 3 14%
Student > Doctoral Student 2 10%
Other 2 10%
Professor 2 10%
Other 4 19%
Unknown 4 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 48%
Biochemistry, Genetics and Molecular Biology 4 19%
Philosophy 1 5%
Chemistry 1 5%
Unknown 5 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 April 2014.
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