Chapter title |
Agrobacterium tumefaciens-Mediated Transient Transformation of Arabidopsis thaliana Leaves
|
---|---|
Chapter number | 8 |
Book title |
Arabidopsis Protocols
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-62703-580-4_8 |
Pubmed ID | |
Book ISBNs |
978-1-62703-579-8, 978-1-62703-580-4
|
Authors |
Silvina Mangano, Cintia Daniela Gonzalez, Silvana Petruccelli |
Abstract |
Transient assays provide a convenient alternative to stable transformation. Compared to the generation of stably transformed plants, agroinfiltration is more rapid, and samples can be analyzed a few days after inoculation. Nevertheless, at difference of tobacco and other plant species, Arabidopsis thaliana remains recalcitrant to routine transient assays. In this chapter, we describe a transient expression assay using simple infiltration of intact Arabidopsis leaves with Agrobacterium tumefaciens carrying a plasmid expressing a reporter fluorescent protein. In this protocol, Agrobacterium aggressiveness was increased by a prolonged treatment in an induction medium deficient in nutrients and containing acetosyringone. Besides, Arabidopsis plants were cultivated in intermediate photoperiod (12 h light-12 h dark) to promote leaf growth. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Chile | 1 | 2% |
United States | 1 | 2% |
Unknown | 63 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 15 | 23% |
Student > Ph. D. Student | 13 | 20% |
Student > Bachelor | 12 | 18% |
Researcher | 11 | 17% |
Unspecified | 2 | 3% |
Other | 4 | 6% |
Unknown | 8 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 31 | 48% |
Biochemistry, Genetics and Molecular Biology | 16 | 25% |
Unspecified | 2 | 3% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Immunology and Microbiology | 1 | 2% |
Other | 2 | 3% |
Unknown | 12 | 18% |