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Single Domain Antibodies

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Cover of 'Single Domain Antibodies'

Table of Contents

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    Book Overview
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    Chapter 1 From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small
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    Chapter 2 Introduction to heavy chain antibodies and derived nanobodies.
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    Chapter 3 Overview and Discovery of IgNARs and Generation of VNARs
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    Chapter 4 Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and Vκ Domain Antibodies
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    Chapter 5 Preparation of a naïve library of camelid single domain antibodies.
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    Chapter 6 Selection by Phage Display of Single Domain Antibodies Specific to Antigens in Their Native Conformation
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    Chapter 7 Semiautomated Panning of Naive Camelidae Libraries and Selection of Single-Domain Antibodies Against Peptide Antigens
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    Chapter 8 Pichia Surface Display: A Tool for Screening Single Domain Antibodies
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    Chapter 9 Bacterial Two Hybrid: A Versatile One-Step Intracellular Selection Method
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    Chapter 10 Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies
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    Chapter 11 Selection of Functional Single Domain Antibody Fragments for Interfering with Protein–Protein Interactions Inside Cells: A “One Plasmid” Mammalian Two-Hybrid System
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    Chapter 12 Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization
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    Chapter 13 Selection of VHHs Under Application Conditions.
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    Chapter 14 Isolation and Characterization of Clostridium difficile Toxin-Specific Single-Domain Antibodies
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    Chapter 15 Selection of VHH Antibody Fragments That Recognize Different Aβ Depositions Using Complex Immune Libraries.
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    Chapter 16 Expression of Single-Domain Antibodies in Bacterial Systems
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    Chapter 17 Expression of VHHs in Saccharomyces cerevisiae
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    Chapter 18 Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells
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    Chapter 19 Production of Camel-Like Antibodies in Plants
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    Chapter 20 Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains
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    Chapter 21 Solubility and Stability Engineering of Human VH Domains
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    Chapter 22 Improvement of Proteolytic Stability Through In Silico Engineering
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    Chapter 23 Single Domain Antibodies
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    Chapter 24 Improvement of Single Domain Antibody Stability by Disulfide Bond Introduction
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    Chapter 25 Characterization of Single-Domain Antibodies with an Engineered Disulfide Bond
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    Chapter 26 Affinity Maturation of Single-Domain Antibodies by Yeast Surface Display
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    Chapter 27 Multivalent Display of Single-Domain Antibodies
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    Chapter 28 Methods for Determining the PK Parameters of AlbudAbs™ and of Long Serum Half-Life Drugs Made Using the AlbudAb™ Technology
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    Chapter 29 Fluorescent Protein Specific Nanotraps to Study Protein–Protein Interactions and Histone-Tail Peptide Binding
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    Chapter 30 Site-Specific Labeling of His-Tagged Nanobodies with 99m Tc: A Practical Guide
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    Chapter 31 Nanobody-Based Chromatin Immunoprecipitation
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    Chapter 32 User-Friendly Expression Plasmids Enable the Fusion of VHHs to Application-Specific Tags
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    Chapter 33 Application of Single-Domain Antibodies in Tumor Histochemistry
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    Chapter 34 Nanobodies as structural probes of protein misfolding and fibril formation.
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    Chapter 35 Molecular Imaging Using Nanobodies: A Case Study
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    Chapter 36 Case Study on Live Cell Apoptosis-Assay Using Lamin-Chromobody Cell-Lines for High-Content Analysis
Attention for Chapter 20: Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains
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Chapter title
Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains
Chapter number 20
Book title
Single Domain Antibodies
Published in
Methods in molecular biology, January 2012
DOI 10.1007/978-1-61779-968-6_20
Pubmed ID
Book ISBNs
978-1-61779-967-9, 978-1-61779-968-6
Authors

Raffi Tonikian, Sachdev S. Sidhu

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 47%
Student > Ph. D. Student 3 18%
Professor 1 6%
Other 1 6%
Student > Master 1 6%
Other 1 6%
Unknown 2 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 41%
Biochemistry, Genetics and Molecular Biology 6 35%
Veterinary Science and Veterinary Medicine 1 6%
Chemical Engineering 1 6%
Unknown 2 12%