Single Domain Antibodies

Chapter 1 From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small

Chapter 2 Introduction to heavy chain antibodies and derived nanobodies.

Chapter 3 Overview and Discovery of IgNARs and Generation of VNARs

Chapter 4 Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and Vκ Domain Antibodies

Chapter 5 Preparation of a naïve library of camelid single domain antibodies.

Chapter 6 Selection by Phage Display of Single Domain Antibodies Specific to Antigens in Their Native Conformation

Chapter 7 Semiautomated Panning of Naive Camelidae Libraries and Selection of Single-Domain Antibodies Against Peptide Antigens

Chapter 8 Pichia Surface Display: A Tool for Screening Single Domain Antibodies

Chapter 9 Bacterial Two Hybrid: A Versatile One-Step Intracellular Selection Method

Chapter 10 Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies

Chapter 11 Selection of Functional Single Domain Antibody Fragments for Interfering with Protein–Protein Interactions Inside Cells: A “One Plasmid” Mammalian Two-Hybrid System

Chapter 12 Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization

Chapter 13 Selection of VHHs Under Application Conditions.

Chapter 14 Isolation and Characterization of Clostridium difficile Toxin-Specific Single-Domain Antibodies

Chapter 15 Selection of VHH Antibody Fragments That Recognize Different Aβ Depositions Using Complex Immune Libraries.

Chapter 16 Expression of Single-Domain Antibodies in Bacterial Systems

Chapter 17 Expression of VHHs in Saccharomyces cerevisiae

Chapter 18 Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells

Chapter 19 Production of Camel-Like Antibodies in Plants

Chapter 20 Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains

Chapter 21 Solubility and Stability Engineering of Human VH Domains

Chapter 22 Improvement of Proteolytic Stability Through In Silico Engineering

Chapter 23 Single Domain Antibodies

Chapter 24 Improvement of Single Domain Antibody Stability by Disulfide Bond Introduction

Chapter 25 Characterization of Single-Domain Antibodies with an Engineered Disulfide Bond

Chapter 26 Affinity Maturation of Single-Domain Antibodies by Yeast Surface Display

Chapter 27 Multivalent Display of Single-Domain Antibodies

Chapter 28 Methods for Determining the PK Parameters of AlbudAbs™ and of Long Serum Half-Life Drugs Made Using the AlbudAb™ Technology

Chapter 29 Fluorescent Protein Specific Nanotraps to Study Protein–Protein Interactions and Histone-Tail Peptide Binding

Chapter 30 Site-Specific Labeling of His-Tagged Nanobodies with 99m Tc: A Practical Guide

Chapter 31 Nanobody-Based Chromatin Immunoprecipitation

Chapter 32 User-Friendly Expression Plasmids Enable the Fusion of VHHs to Application-Specific Tags

Chapter 33 Application of Single-Domain Antibodies in Tumor Histochemistry

Chapter 34 Nanobodies as structural probes of protein misfolding and fibril formation.

Chapter 35 Molecular Imaging Using Nanobodies: A Case Study

Chapter 36 Case Study on Live Cell Apoptosis-Assay Using Lamin-Chromobody Cell-Lines for High-Content Analysis