| Chapter title |
The Stem Cell Laboratory: Design, Equipment, and Oversight
|
|---|---|
| Chapter number | 1 |
| Book title |
Human Pluripotent Stem Cells
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-61779-201-4_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-200-7, 978-1-61779-201-4, 978-1-61779-200-7, 978-1-61779-201-4
|
| Authors |
Robin L. Wesselschmidt, Philip H. Schwartz |
| Abstract |
This chapter describes some of the major issues to be considered when setting up a laboratory for the culture of human pluripotent stem cells (hPSCs). The process of establishing a hPSC laboratory can be divided into two equally important parts. One is completely administrative and includes developing protocols, seeking approval, and establishing reporting processes and documentation. The other part of establishing a hPSC laboratory involves the physical plant and includes design, equipment and personnel. Proper planning of laboratory operations and proper design of the physical layout of the stem cell laboratory so that meets the scope of planned operations is a major undertaking, but the time spent upfront will pay long-term returns in operational efficiency and effectiveness. A well-planned, organized, and properly equipped laboratory supports research activities by increasing efficiency and reducing lost time and wasted resources. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Portugal | 1 | 1% |
| Unknown | 68 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 10% |
| Student > Bachelor | 7 | 10% |
| Other | 6 | 9% |
| Professor | 6 | 9% |
| Student > Doctoral Student | 5 | 7% |
| Other | 23 | 33% |
| Unknown | 16 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 19% |
| Agricultural and Biological Sciences | 13 | 19% |
| Medicine and Dentistry | 6 | 9% |
| Immunology and Microbiology | 5 | 7% |
| Engineering | 4 | 6% |
| Other | 12 | 17% |
| Unknown | 17 | 24% |