Therapeutic Proteins

Chapter 1 Therapeutic proteins.

Chapter 2 Synthetic antibody libraries.

Chapter 3 The Construction of “Phylomer” Peptide Libraries as a Rich Source of Potent Inhibitors of Protein/Protein Interactions

Chapter 4 Ribosome Display and Screening for Protein Therapeutics

Chapter 5 Yeast display of engineered antibody domains.

Chapter 6 Expression, Purification, and Characterization of Engineered Antibody CH2 and VH Domains.

Chapter 7 Engineering of Affibody Molecules for Therapy and Diagnostics

Chapter 8 Protein Design for Diversity of Sequences and Conformations Using Dead-End Elimination

Chapter 9 Design and Generation of DVD-Ig™ Molecules for Dual-Specific Targeting

Chapter 10 Engineering and Expression of Bibody and Tribody Constructs in Mammalian Cells and in the Yeast Pichia pastoris

Chapter 11 Use of E. coli for the Production of a Single Protein

Chapter 12 Folding Engineering Strategies for Efficient Membrane Protein Production in E. coli

Chapter 13 Transient Expression Technologies: Past, Present, and Future

Chapter 14 Stable Transfection Pools for Large Quantity of Protein Production

Chapter 15 Mammalian Stable Expression of Biotherapeutics

Chapter 16 Transgenic Expression of Therapeutic Proteins in Arabidopsis thaliana Seed

Chapter 17 Methods for Chromatographic Removal of Endotoxin

Chapter 18 Effectiveness of various processing steps for viral clearance of therapeutic proteins: database analyses of commonly used steps.

Chapter 19 High-Throughput Quantitative N-Glycan Analysis of Glycoproteins

Chapter 20 High-Throughput Multimodal Strong Anion Exchange Purification and N-Glycan Characterization of Endogenous Glycoprotein Expressed in Glycoengineered Pichia pastoris

Chapter 21 Databases and tools in glycobiology.

Chapter 22 Characterization of PEGylated Biopharmaceutical Products by LC/MS and LC/MS/MS

Chapter 23 Identification of Asp Isomerization in Proteins by 18 O Labeling and Tandem Mass Spectrometry

Chapter 24 Monitoring of Subvisible Particles in Therapeutic Proteins

Chapter 25 Size-Exclusion Chromatography with Multi-angle Light Scattering for Elucidating Protein Aggregation Mechanisms

Chapter 26 Computational Methods to Predict Therapeutic Protein Aggregation

Chapter 27 Coarse-Grained Simulations of Protein Aggregation

Chapter 28 Chitosan-based nanoparticles as delivery systems of therapeutic proteins.

Chapter 29 Challenges in the Development and Manufacturing of Antibody–Drug Conjugates