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The SH-3 and SH-4 Antibodies Recognize Distinct Epitopes on CD73 from Human Mesenchymal Stem Cells

Overview of attention for article published in Biochemical & Biophysical Research Communications, November 2001
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (83rd percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Mentioned by

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32 patents

Citations

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198 Dimensions

Readers on

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95 Mendeley
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Title
The SH-3 and SH-4 Antibodies Recognize Distinct Epitopes on CD73 from Human Mesenchymal Stem Cells
Published in
Biochemical & Biophysical Research Communications, November 2001
DOI 10.1006/bbrc.2001.6013
Pubmed ID
Authors

Frank Barry, Raymond Boynton, Mary Murphy, Joseph Zaia

Abstract

Mesenchymal stem cells (MSCs) are pluripotent cells in the bone marrow that have the capacity to differentiate along a number of connective tissue lineages, including cartilage, bone, adipose tissue, and stroma. The SH-3 and SH-4 monoclonal antibodies recognize epitopes present on the surface of human MSCs. This study describes the isolation and characterization of the antigen that is recognized by these antibodies. A protein of molecular weight approximately 67 kDa was immunoprecipitated from a solubilized membrane preparation of human MSCs using the SH-3 antibody. Analysis of peptides derived from this protein by mass spectrometry and sequencing identified it as CD73 (ecto-5'-nucleotidase). The SH-4 antibody was also shown to react with purified bovine CD73 by immunoblotting, but the SH-3 antibody failed to react with the bovine protein. These results indicate that both SH-3 and SH-4 epitopes are present on CD73, but they are distinct. CD73, present in lymphoid tissue, plays a role in the activation of B-lymphocytes and in signal transduction in the hematopoietic compartment of bone marrow. The role that CD73 may play in bone marrow stromal interactions and in the differentiation of MSCs is discussed.

Mendeley readers

The data shown below were compiled from readership statistics for 95 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 3%
United Kingdom 2 2%
Czechia 1 1%
Japan 1 1%
France 1 1%
Unknown 87 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 24 25%
Student > Ph. D. Student 17 18%
Student > Bachelor 11 12%
Student > Master 10 11%
Student > Postgraduate 8 8%
Other 22 23%
Unknown 3 3%
Readers by discipline Count As %
Agricultural and Biological Sciences 43 45%
Medicine and Dentistry 19 20%
Biochemistry, Genetics and Molecular Biology 14 15%
Engineering 4 4%
Veterinary Science and Veterinary Medicine 2 2%
Other 3 3%
Unknown 10 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2021.
All research outputs
#1,859,482
of 17,709,629 outputs
Outputs from Biochemical & Biophysical Research Communications
#339
of 23,730 outputs
Outputs of similar age
#48,127
of 287,581 outputs
Outputs of similar age from Biochemical & Biophysical Research Communications
#10
of 243 outputs
Altmetric has tracked 17,709,629 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 23,730 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 287,581 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 243 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.