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Sirtuins

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Cover of 'Sirtuins'

Table of Contents

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    Book Overview
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    Chapter 1 Introduction: sirtuins in aging and diseases.
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    Chapter 2 Sirtuins in Yeast: Phenotypes and Tools
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    Chapter 3 C. elegans Sirtuins.
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    Chapter 4 Genetic and Biochemical Tools for Investigating Sirtuin Function in Drosophila melanogaster.
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    Chapter 5 Generating Mammalian sirtuin tools for protein-interaction analysis.
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    Chapter 6 Mass spectrometry-based detection of protein acetylation.
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    Chapter 7 SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome.
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    Chapter 8 Targeted Quantitation of Acetylated Lysine Peptides by Selected Reaction Monitoring Mass Spectrometry
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    Chapter 9 Identification of deacetylase substrates with the biotin switch approach.
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    Chapter 10 Assaying Chromatin Sirtuins
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    Chapter 11 Measurement of sirtuin enzyme activity using a substrate-agnostic fluorometric nicotinamide assay.
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    Chapter 12 Detecting Sirtuin-Catalyzed Deacylation Reactions Using 32 P-Labeled NAD and Thin-Layer Chromatography
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    Chapter 13 Chemical Acetylation and Deacetylation
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    Chapter 14 Accurate Measurement of Nicotinamide Adenine Dinucleotide (NAD + ) with High-Performance Liquid Chromatography
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    Chapter 15 In Vivo Measurement of the Acetylation State of Sirtuin Substrates as a Proxy for Sirtuin Activity
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    Chapter 16 Oxygen flux analysis to understand the biological function of sirtuins.
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    Chapter 17 The Emerging Links Between Sirtuins and Autophagy
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    Chapter 18 Methods to Study the Role of Sirtuins in Genome Stability
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    Chapter 19 Circadian Measurements of Sirtuin Biology
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    Chapter 20 Utilizing Calorie Restriction to Evaluate the Role of Sirtuins in Healthspan and Lifespan of Mice
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    Chapter 21 Erratum: Sirtuins: Methods and Protocols
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