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A duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae

Overview of attention for article published in Virology Journal, January 2015
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (57th percentile)

Mentioned by

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3 tweeters
facebook
1 Facebook page

Citations

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4 Dimensions

Readers on

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21 Mendeley
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Title
A duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae
Published in
Virology Journal, January 2015
DOI 10.1186/s12985-015-0255-y
Pubmed ID
Authors

Li Huang, Zhixun Xie, Liji Xie, Xianwen Deng, Zhiqin Xie, Sisi Luo, Jiaoling Huang, Tingting Zeng, Jiaxun Feng

Abstract

Infectious arthritis in broilers represents an economic and health problem, resulting in severe losses due to retarded growth and downgrading at the slaughterhouse. The most common agents associated with cases of infectious arthritis in poultry are avian reovirus (ARV) and Mycoplasma synoviae (MS). The accurate differentiation and rapid diagnosis of ARV and MS are essential prerequisites for the effective control and prevention of these avian pathogens in poultry flocks. This study thus aimed to develop and validate a duplex real-time PCR assay for the simultaneous detection and quantification of ARV and MS. Specific primers and probes for each pathogen were designed to target the special sequence of the ARV σC gene or the MS phase-variable surface lipoprotein hemagglutinin (vlhA) gene. A duplex real-time PCR assay was developed, and the reaction conditions were optimized for the rapid detection and quantification of ARV and MS. The duplex real-time PCR assay was capable of ARV- and MS-specific detection without cross-reaction with other non-targeted avian pathogens. The sensitivity of this assay was 2 × 10(1) copies for a recombinant plasmid containing ARV σC or MS vlhA gene, and 100 times higher than that of conventional PCR. This newly developed PCR assay was also reproducible and stable. All tested field samples of ARV and/or MS were detectable with this duplex real-time PCR assay compared with pathogen isolation and identification as well as serological tests. This duplex real-time PCR assay is highly specific, sensitive and reproducible and thus could provide a rapid, specific and sensitive diagnostic tool for the simultaneous detection of ARV and MS in poultry flocks. The assay will be useful not only for clinical diagnostics and disease surveillance but also for the efficient control and prevention of ARV and MS infections.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Greece 1 5%
Peru 1 5%
Unknown 19 90%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 24%
Researcher 4 19%
Student > Bachelor 2 10%
Student > Doctoral Student 2 10%
Professor 2 10%
Other 3 14%
Unknown 3 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 38%
Veterinary Science and Veterinary Medicine 4 19%
Biochemistry, Genetics and Molecular Biology 2 10%
Medicine and Dentistry 2 10%
Immunology and Microbiology 1 5%
Other 1 5%
Unknown 3 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 February 2015.
All research outputs
#2,234,043
of 5,021,372 outputs
Outputs from Virology Journal
#597
of 1,360 outputs
Outputs of similar age
#74,737
of 180,855 outputs
Outputs of similar age from Virology Journal
#42
of 55 outputs
Altmetric has tracked 5,021,372 research outputs across all sources so far. This one has received more attention than most of these and is in the 54th percentile.
So far Altmetric has tracked 1,360 research outputs from this source. They receive a mean Attention Score of 3.3. This one has gotten more attention than average, scoring higher than 51% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 180,855 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.
We're also able to compare this research output to 55 others from the same source and published within six weeks on either side of this one. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.