| Chapter title |
USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories.
|
|---|---|
| Chapter number | 13 |
| Book title |
Plant Secondary Metabolism Engineering
|
| Published in |
Methods in molecular biology, June 2010
|
| DOI | 10.1007/978-1-60761-723-5_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-60761-722-8, 978-1-60761-723-5
|
| Authors |
Nour-Eldin HH, Geu-Flores F, Halkier BA, Hussam H. Nour-Eldin, Fernando Geu-Flores, Barbara A. Halkier, Nour-Eldin, Hussam H., Geu-Flores, Fernando, Halkier, Barbara A. |
| Abstract |
The explosive development of the field of molecular biology has led to the need for simpler and more efficient cloning techniques. These requirements are elegantly met by the ligation-free cloning technique called USER cloning. USER cloning is suitable not only for everyday and high-throughput cloning but also for the one-step construction of complex DNA constructs, which can be achieved in a variant called USER fusion. In this chapter, we present a general protocol for converting any vector into a USER-compatible vector, together with protocols for both USER cloning and USER fusion. |
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|---|---|---|
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| Unknown | 292 | 97% |
Demographic breakdown
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|---|---|---|
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| Student > Bachelor | 58 | 19% |
| Researcher | 44 | 15% |
| Student > Master | 36 | 12% |
| Student > Postgraduate | 7 | 2% |
| Other | 22 | 7% |
| Unknown | 67 | 22% |
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|---|---|---|
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| Other | 12 | 4% |
| Unknown | 73 | 24% |