↓ Skip to main content

Efficient gene editing in Neurospora crassa with CRISPR technology

Overview of attention for article published in Fungal Biology and Biotechnology, July 2015
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (66th percentile)

Mentioned by

twitter
1 tweeter
patent
2 patents

Citations

dimensions_citation
73 Dimensions

Readers on

mendeley
176 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Efficient gene editing in Neurospora crassa with CRISPR technology
Published in
Fungal Biology and Biotechnology, July 2015
DOI 10.1186/s40694-015-0015-1
Pubmed ID
Authors

Toru Matsu-ura, Mokryun Baek, Jungin Kwon, Christian Hong

Abstract

Efficient gene editing is a critical tool for investigating molecular mechanisms of cellular processes and engineering organisms for numerous purposes ranging from biotechnology to medicine. Recently developed RNA-guided CRISPR/Cas9 technology has been used for efficient gene editing in various organisms, but has not been tested in a model filamentous fungus, Neurospora crassa. In this report, we demonstrate efficient gene replacement in a model filamentous fungus, Neurospora crassa, with the CRISPR/Cas9 system. We utilize Cas9 endonuclease and single crRNA:tracrRNA chimeric guide RNA (gRNA) to: (1) replace the endogenous promoter of clr-2 with the β-tubulin promoter, and (2) introduce a codon optimized fire fly luciferase under the control of the gsy-1 promoter at the csr-1 locus. CLR-2 is one of the core transcription factors that regulate the expression of cellulases, and GSY-1 regulates the conversion of glucose into glycogen. We show that the β-tubulin promoter driven clr-2 strain shows increased expression of cellulases, and gsy-1-luciferase reporter strain can be easily screened with a bioluminescence assay. CRISPR/Cas9 system works efficiently in Neurospora crassa, which may be adapted to Neurospora natural isolates and other filamentous fungi. It will be beneficial for the filamentous fungal research community to take advantage of CRISPR/Cas9 tool kits that enable genetic perturbations including gene replacement and insertions.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 176 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 <1%
Chile 1 <1%
Italy 1 <1%
Brazil 1 <1%
Mexico 1 <1%
Denmark 1 <1%
United States 1 <1%
Unknown 169 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 51 29%
Student > Master 33 19%
Researcher 25 14%
Student > Bachelor 22 13%
Student > Doctoral Student 10 6%
Other 24 14%
Unknown 11 6%
Readers by discipline Count As %
Agricultural and Biological Sciences 89 51%
Biochemistry, Genetics and Molecular Biology 49 28%
Engineering 5 3%
Immunology and Microbiology 4 2%
Chemistry 3 2%
Other 11 6%
Unknown 15 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 September 2019.
All research outputs
#3,669,829
of 13,960,267 outputs
Outputs from Fungal Biology and Biotechnology
#26
of 78 outputs
Outputs of similar age
#92,167
of 276,132 outputs
Outputs of similar age from Fungal Biology and Biotechnology
#1
of 1 outputs
Altmetric has tracked 13,960,267 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 78 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one has gotten more attention than average, scoring higher than 66% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 276,132 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them