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Aurora-B Phosphorylation in Vitro Identifies a Residue of Survivin That Is Essential for Its Localization and Binding to Inner Centromere Protein (INCENP) in Vivo

Overview of attention for article published in Journal of Biological Chemistry, November 2003
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • High Attention Score compared to outputs of the same age and source (88th percentile)

Mentioned by

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3 patents
wikipedia
1 Wikipedia page

Citations

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103 Dimensions

Readers on

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60 Mendeley
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Title
Aurora-B Phosphorylation in Vitro Identifies a Residue of Survivin That Is Essential for Its Localization and Binding to Inner Centromere Protein (INCENP) in Vivo
Published in
Journal of Biological Chemistry, November 2003
DOI 10.1074/jbc.m311299200
Pubmed ID
Authors

Sally P. Wheatley, Alexander J. Henzing, Helen Dodson, Walid Khaled, William C. Earnshaw

Abstract

The chromosomal passengers, aurora-B kinase, inner centromere protein (INCENP), and survivin, are essential proteins that have been implicated in the regulation of metaphase chromosome alignment, spindle checkpoint function, and cytokinesis. All three share a common pattern of localization, and it was recently demonstrated that aurora-B, INCENP, and survivin are present in a complex in Xenopus eggs and Saccharomyces cerevisiae. The presence of aurora-B kinase in the complex and its ability to bind the other components directly suggest that INCENP and survivin could potentially be aurora-B substrates. This hypothesis was recently proven for INCENP in vitro. Here we report that human survivin is specifically phosphorylated in vitro by aurora-B kinase at threonine 117 in its carboxyl alpha-helical coil. Mutation of threonine 117 to alanine prevents survivin phosphorylation by aurora-B in vitro but does not alter its localization in HeLa cells. By contrast, a phospho-mimic, in which threonine 117 was mutated to glutamic acid, was unable to localize correctly at any stage in mitosis. Mutation at threonine 117 also prevented immunoprecipitation of INCENP with survivin in vivo. These data suggest that phosphorylation of survivin at threonine 117 by aurora-B may regulate targeting of survivin, and possibly the entire passenger complex, in mammals.

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 2%
Germany 1 2%
Brazil 1 2%
Unknown 57 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 30%
Researcher 13 22%
Student > Master 9 15%
Professor 4 7%
Student > Bachelor 3 5%
Other 7 12%
Unknown 6 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 27 45%
Biochemistry, Genetics and Molecular Biology 20 33%
Medicine and Dentistry 4 7%
Unspecified 1 2%
Computer Science 1 2%
Other 1 2%
Unknown 6 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 January 2015.
All research outputs
#2,132,334
of 12,365,423 outputs
Outputs from Journal of Biological Chemistry
#6,154
of 53,821 outputs
Outputs of similar age
#53,014
of 269,291 outputs
Outputs of similar age from Journal of Biological Chemistry
#50
of 438 outputs
Altmetric has tracked 12,365,423 research outputs across all sources so far. Compared to these this one has done well and is in the 78th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 53,821 research outputs from this source. They receive a mean Attention Score of 4.2. This one has gotten more attention than average, scoring higher than 74% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 269,291 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 438 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.