Title |
Anti-Ephrin Type-B Receptor 2 (EphB2) and Anti-Three Prime Histone mRNA EXonuclease 1 (THEX1) Autoantibodies in Scleroderma and Lupus
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Published in |
PLOS ONE, September 2016
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DOI | 10.1371/journal.pone.0160283 |
Pubmed ID | |
Authors |
Doua F. Azzouz, Gabriel V. Martin, Fanny Arnoux, Nathalie Balandraud, Thierry Martin, Sylvain Dubucquoi, Eric Hachulla, Dominique Farge-Bancel, Kiet Tiev, Jean Cabane, Nathalie Bardin, Laurent Chiche, Marielle Martin, Eléonore C. Caillet, Sami B. Kanaan, Jean Robert Harlé, Brigitte Granel, Elisabeth Diot, Jean Roudier, Isabelle Auger, Nathalie C. Lambert |
Abstract |
In a pilot ProtoArray analysis, we identified 6 proteins out of 9483 recognized by autoantibodies (AAb) from patients with systemic sclerosis (SSc). We further investigated the 6 candidates by ELISA on hundreds of controls and patients, including patients with Systemic Lupus Erythematosus (SLE), known for high sera reactivity and overlapping AAb with SSc. Only 2 of the 6 candidates, Ephrin type-B receptor 2 (EphB2) and Three prime Histone mRNA EXonuclease 1 (THEX1), remained significantly recognized by sera samples from SSc compared to controls (healthy or with rheumatic diseases) with, respectively, 34% versus 14% (P = 2.10-4) and 60% versus 28% (P = 3.10-8). Above all, EphB2 and THEX1 revealed to be mainly recognized by SLE sera samples with respectively 56%, (P = 2.10-10) and 82% (P = 5.10-13). As anti-EphB2 and anti-THEX1 AAb were found in both diseases, an epitope mapping was realized on each protein to refine SSc and SLE diagnosis. A 15-mer peptide from EphB2 allowed to identify 35% of SLE sera samples (N = 48) versus only 5% of any other sera samples (N = 157), including SSc sera samples. AAb titers were significantly higher in SLE sera (P<0.0001) and correlated with disease activity (p<0.02). We could not find an epitope on EphB2 protein for SSc neither on THEX1 for SSc or SLE. We showed that patients with SSc or SLE have AAb against EphB2, a protein involved in angiogenesis, and THEX1, a 3'-5' exoribonuclease involved in histone mRNA degradation. We have further identified a peptide from EphB2 as a specific and sensitive tool for SLE diagnosis. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Doctoral Student | 3 | 18% |
Student > Master | 2 | 12% |
Student > Postgraduate | 2 | 12% |
Student > Ph. D. Student | 2 | 12% |
Researcher | 2 | 12% |
Other | 2 | 12% |
Unknown | 4 | 24% |
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Medicine and Dentistry | 8 | 47% |
Philosophy | 1 | 6% |
Agricultural and Biological Sciences | 1 | 6% |
Nursing and Health Professions | 1 | 6% |
Unknown | 6 | 35% |