Chapter title |
Chronic Myeloid Leukemia
|
---|---|
Chapter number | 11 |
Book title |
Chronic Myeloid Leukemia
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-4011-0_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-4009-7, 978-1-4939-4011-0
|
Authors |
Anders, Lars, Li, Zhaodong, Lars Anders, Zhaodong Li |
Abstract |
Many transcription factors, chromatin-associated proteins and regulatory DNA elements are genetically and/or epigenetically altered in cancer, including Chronic Myeloid Leukemia (CML). This leads to deregulation of transcription that is often causally linked to the tumorigenic state. Chromatin-immunoprecipitation coupled with massively parallel DNA sequencing (ChIP-seq) is the key technology to study transcription as it allows in vivo whole-genome mapping of epigenetic modifications and interactions of proteins with DNA or chromatin. However, numerous DNA/chromatin-binding proteins, including EZH2, remain difficult to "ChIP," thus yielding genome-wide binding maps of only suboptimal quality. Here, we describe a ChIP-seq protocol optimized for high-quality protein-genome binding maps that have proven especially useful for studying difficult to 'ChIP' transcription regulatory factors in Chronic Myeloid Leukemia (CML) and related malignancies. |
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