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Mendeley readers
Chapter title |
Quantitative Proteomics Analysis of Leukemia Cells
|
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Chapter number | 12 |
Book title |
Chronic Myeloid Leukemia
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-4011-0_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-4009-7, 978-1-4939-4011-0
|
Authors |
Sebastian Halbach, Jörn Dengjel, Tilman Brummer, Halbach, Sebastian, Dengjel, Jörn, Brummer, Tilman |
Abstract |
Chronic myeloid leukemia (CML) is driven by the oncogenic fusion kinase Bcr-Abl, which organizes its own signaling network with various proteins. These proteins, their interactions, and their role in relevant signaling pathways can be analyzed by quantitative mass spectrometry (MS) approaches in various models systems, e.g., in cell culture models. In this chapter, we describe in detail immunoprecipitations and quantitative proteomics analysis using stable isotope labeling by amino acids in cell culture (SILAC) of components of the Bcr-Abl signaling pathway in the human CML cell line K562. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 20% |
Professor > Associate Professor | 1 | 20% |
Lecturer > Senior Lecturer | 1 | 20% |
Unknown | 2 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 20% |
Biochemistry, Genetics and Molecular Biology | 1 | 20% |
Chemistry | 1 | 20% |
Unknown | 2 | 40% |