↓ Skip to main content

In Situ Hybridization Protocols

Overview of attention for book
Cover of 'In Situ Hybridization Protocols'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Fixation/Permeabilization Procedure for mRNA In Situ Hybridization of Zebrafish Whole-Mount Oocytes, Embryos, and Larvae
  3. Altmetric Badge
    Chapter 2 MicroRNA In Situ Hybridization on Whole-Mount Preimplantation Embryos
  4. Altmetric Badge
    Chapter 3 Whole-Mount In Situ Hybridization (WISH) Optimized for Gene Expression Analysis in Mouse Embryos and Embryoid Bodies
  5. Altmetric Badge
    Chapter 4 Whole-Mount In Situ Hybridization Using DIG-Labeled Probes in Planarian.
  6. Altmetric Badge
    Chapter 5 In Situ Hybridization on Whole-Mount Zebrafish Embryos and Young Larvae
  7. Altmetric Badge
    Chapter 6 LNA-Based In Situ Hybridization Detection of mRNAs in Embryos
  8. Altmetric Badge
    Chapter 7 Chromogen Detection of microRNA in Frozen Clinical Tissue Samples Using LNA™ Probe Technology.
  9. Altmetric Badge
    Chapter 8 MicroRNA In Situ Hybridization in Tissue Microarrays.
  10. Altmetric Badge
    Chapter 9 Fluorescence In Situ Hybridization for Detection of Small RNAs on Frozen Tissue Sections.
  11. Altmetric Badge
    Chapter 10 Sensitive and Specific In Situ Hybridization for Early Drug Discovery
  12. Altmetric Badge
    Chapter 11 Zinc-Based Fixation for High-Sensitivity In Situ Hybridization: A Nonradioactive Colorimetric Method for the Detection of Rare Transcripts on Tissue Sections
  13. Altmetric Badge
    Chapter 12 Dual-Color Ultrasensitive Bright-Field RNA In Situ Hybridization with RNAscope.
  14. Altmetric Badge
    Chapter 13 Fully Automated Fluorescence-Based Four-Color Multiplex Assay for Co-detection of MicroRNA and Protein Biomarkers in Clinical Tissue Specimens.
  15. Altmetric Badge
    Chapter 14 Multiplexed miRNA Fluorescence In Situ Hybridization for Formalin-Fixed Paraffin-Embedded Tissues.
  16. Altmetric Badge
    Chapter 15 Simultaneous detection of nuclear and cytoplasmic RNA variants utilizing stellaris(®) RNA fluorescence in situ hybridization in adherent cells.
  17. Altmetric Badge
    Chapter 16 Quantitative Ultrasensitive Bright-Field RNA In Situ Hybridization with RNAscope
  18. Altmetric Badge
    Chapter 17 Identification of Low-Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epithelia
  19. Altmetric Badge
    Chapter 18 In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes
  20. Altmetric Badge
    Chapter 19 In Situ Hybridization Freeze-Assisted Punches (IFAP): Technique for Liquid-Based Tissue Extraction from Thin Slide-Mounted Sections for DNA Methylation Analysis.
  21. Altmetric Badge
    Chapter 20 miRNA Detection at Single-Cell Resolution Using Microfluidic LNA Flow-FISH.
  22. Altmetric Badge
    Chapter 21 PNA-Based Fluorescence In Situ Hybridization for Identification of Bacteria in Clinical Samples.
Attention for Chapter 7: Chromogen Detection of microRNA in Frozen Clinical Tissue Samples Using LNA™ Probe Technology.
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
4 Dimensions

Readers on

mendeley
11 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Chromogen Detection of microRNA in Frozen Clinical Tissue Samples Using LNA™ Probe Technology.
Chapter number 7
Book title
In Situ Hybridization Protocols
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-1459-3_7
Pubmed ID
Book ISBNs
978-1-4939-1458-6, 978-1-4939-1459-3
Authors

Boye Schnack Nielsen, Trine Møller, Kim Holmstrøm, Nielsen, Boye Schnack, Møller, Trine, Holmstrøm, Kim

Abstract

Specific chromogen- and fluorescence-based detection of microRNA by in situ hybridization (ISH) in formalin-fixed and paraffin-embedded (FFPE) tissue sections has been facilitated by locked nucleic acid (LNA)-based probe technology and can be performed within a single working day. In the current method paper, we present a similar simple 1-day ISH method developed for cryostat sections obtained from clinical cryo-embedded tissue samples. The presented chromogen-based ISH method does not involve proteolytic pretreatment, which is mandatory for FFPE sections, but still retains a sensitivity level similar to that obtained in FFPE sections. The LNA-based ISH method is not only applicable in situations where only access to cryo-embedded material is possible, but it also has a potential use if combining microRNA ISH with immunohistochemistry in double fluorescence staining with antibodies not being compatible with proteolytic predigestion.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 36%
Researcher 3 27%
Professor > Associate Professor 1 9%
Unspecified 1 9%
Unknown 2 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 36%
Agricultural and Biological Sciences 2 18%
Unspecified 1 9%
Unknown 4 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 September 2014.
All research outputs
#20,236,620
of 22,763,032 outputs
Outputs from Methods in molecular biology
#9,865
of 13,088 outputs
Outputs of similar age
#264,836
of 305,294 outputs
Outputs of similar age from Methods in molecular biology
#405
of 597 outputs
Altmetric has tracked 22,763,032 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,088 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,294 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 597 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.