Chapter title |
Chromogen Detection of microRNA in Frozen Clinical Tissue Samples Using LNA™ Probe Technology.
|
---|---|
Chapter number | 7 |
Book title |
In Situ Hybridization Protocols
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-1459-3_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1458-6, 978-1-4939-1459-3
|
Authors |
Boye Schnack Nielsen, Trine Møller, Kim Holmstrøm, Nielsen, Boye Schnack, Møller, Trine, Holmstrøm, Kim |
Abstract |
Specific chromogen- and fluorescence-based detection of microRNA by in situ hybridization (ISH) in formalin-fixed and paraffin-embedded (FFPE) tissue sections has been facilitated by locked nucleic acid (LNA)-based probe technology and can be performed within a single working day. In the current method paper, we present a similar simple 1-day ISH method developed for cryostat sections obtained from clinical cryo-embedded tissue samples. The presented chromogen-based ISH method does not involve proteolytic pretreatment, which is mandatory for FFPE sections, but still retains a sensitivity level similar to that obtained in FFPE sections. The LNA-based ISH method is not only applicable in situations where only access to cryo-embedded material is possible, but it also has a potential use if combining microRNA ISH with immunohistochemistry in double fluorescence staining with antibodies not being compatible with proteolytic predigestion. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 36% |
Researcher | 3 | 27% |
Professor > Associate Professor | 1 | 9% |
Unspecified | 1 | 9% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 36% |
Agricultural and Biological Sciences | 2 | 18% |
Unspecified | 1 | 9% |
Unknown | 4 | 36% |